1 20 Pierce EZ-Link PEO-Iodoacetyl Biotin 6 56 N-trideuteriumacetoxy 9 60 N-(4-trimethylammoniumbutanoxy)-NHS 13 4 Carboxyamidomethylation 11 6 Carboxymethylation 24 121 glycineglycine 20 108 CysNEM 28 123 Harbury glyco-ICAT C12 29 124 Harbury glyco-ICAT C13 32 126 3,3-Dithio-bis-(sulfosuccinimidyl)propionate 49 135 C14:2 fatty acylation 47 134 C14:1 acylation 48 134 myristoyl with one double bond 50 135 myristoyl with 2 double bonds 52 52 homoarginine 56 140 Decarboxylation of C-terminus as reaction inside the mass spectrometer 57 159 chitobiose core 238 332 Thiophos-biotin disulfide 64 17 Dimethylacrylamide, DMA 66 171 NBS reagent heavy 123 193 Double O18 (C-term) 81 176 Butylated Hydroxytoluene 83 178 b-elimination thiol derivatization 182 184 heavy tyrosine 118 185 heavy phosphotyrosine 96 186 HPG arginine 97 187 2 HPG arginine 184 206 Acrolein56 183 207 Acrolein38 185 208 Acrolein76 187 209 Acrolein112 186 205 Acrolein94, FDP 153 258 H2 18O Alkaline Phosphatase 181 259 heavy lysine 165 261 N-Terminal/lysine sulfonation 173 262 Trideuteration 174 264 b-elimination PET derivatisation 172 94 2-methoxy-4,5-dihydro-1H-imidazole derivative 171 95 2-methoxy-4,5-dihydro-1H-imidazole derivative 233 286 C-Terminal/Glutamate/Aspartate sulfonation 235 285 C-Terminal/Glutamate/Aspartate sulfonation 189 272 CAF 198 292 UV induced cross-link product of Iodo-U-amp with WFY 200 293 3-thiopropanoyl moiety from reduced DSP crosslinker or NHS-SS-biotin, modified with Iodoacetamide 206 295 deoxyhexose 207 296 N-Terminal/arginine sulfonation 208 297 Guanidination followed by N-Terminal/Lysine sulfonation 217 301 mBromobimane 216 302 Vitamin k3 (Q) 225 318 MDA62 224 319 MDA54 227 324 DTBP 253 325 10-fluoroethoxyphosphinyl-N-(biotinamidopentyl)decanamide 251 340 bromotryptophan 252 333 6-N-biotinylaminohexyl isopropyl phosphorofluoridate 1 - 0 0 0 0 0 2 A Ala Alanine 5 3 1 1 0 3 R Arg Arginine 12 6 4 1 0 4 N Asn Asparagine 6 4 2 2 0 5 D Asp Aspartic acid 5 4 1 3 0 6 C Cys Cysteine 5 3 1 1 1 7 E Glu Glutamic acid 7 5 1 3 0 8 Q Gln Glutamine 8 5 2 2 0 9 G Gly Glycine 3 2 1 1 0 10 H His Histidine 7 6 3 1 0 11 I Ile Isoleucine 11 6 1 1 0 12 L Leu Leucine 11 6 1 1 0 13 K Lys Lysine 12 6 2 1 0 14 M Met Methionine 9 5 1 1 1 15 F Phe Phenylalanine 9 9 1 1 0 16 P Pro Proline 7 5 1 1 0 17 S Ser Serine 5 3 1 2 0 18 T Thr Threonine 7 4 1 2 0 19 W Trp Tryptophan 10 11 2 1 0 20 Y Tyr Tyrosine 9 9 1 2 0 21 V Val Valine 9 5 1 1 0 22 N-term N-term N-term 1 0 0 0 0 23 C-term C-term C-term 1 0 0 1 0 1 2 H 1 2 3 C 1 3 4 N 1 4 5 O 1 5 6 P 1 6 7 S 1 7 8 H2 1 8 9 O18 1 9 10 F 1 10 11 Na 1 11 12 Se 1 12 13 H 10 13 13 C 6 14 13 O 5 15 14 C 8 16 14 H 13 17 14 N 1 18 14 O 5 19 15 C 2 20 15 H 2 21 15 O 1 22 16 C 6 23 16 H 10 24 16 O 4 25 17 C 6 26 17 H 8 27 17 O 6 28 18 C 9 29 18 H 14 30 18 O 8 31 19 C 8 32 19 H 12 33 19 O 7 34 20 C 1 35 20 H 2 36 21 C 11 37 21 H 17 38 21 N 1 39 21 O 8 40 22 C 11 41 22 H 17 42 22 N 1 43 22 O 9 44 23 H 2 45 23 O 1 46 24 H 1 47 24 P 1 48 24 O 3 49 25 S 1 50 25 O 3 51 26 C 5 52 26 H 8 53 26 O 4 54 27 Li 1 55 28 C13 1 56 29 N15 1 57 30 Cl 1 58 31 K 1 59 32 Ca 1 60 33 Fe 1 61 34 Ni 1 62 35 Zn 1 63 36 Br 1 64 37 Ag 1 65 38 Hg 1 66 39 Au 1 67 40 I 1 1 - 2 H Hydrogen 3 C Carbon 4 N Nitrogen 5 O Oxygen 6 P Phosphorous 7 S Sulphur 8 H2 Deuterium 9 O18 Oxygen 18 10 F Fluorine 11 Na Sodium 12 Se Selenium 13 Hex Hexose 14 HexNAc N-Acetyl Hexosamine 15 Ac Acetate 16 dHex Deoxy-hexose 17 HexA Hexosamine 18 Kdn 3-deoxy-d-glycero-D-galacto-nonulosonic acid 19 Kdo 2-keto-3-deoxyoctulosonic acid 20 Me Methyl 21 NeuAc N-acetyl neuraminic acid 22 NeuGc N-glycoyl neuraminic acid 23 Water Water 24 Phos Phosphate 25 Sulf Sulfate 26 Pent Pentose 27 Li Lithium 28 C13 Carbon 13 29 N15 Nitrogen 15 30 Cl Chlorine 31 K Potassium 32 Ca Calcium 33 Fe Iron 34 Ni Nickel 35 Zn Zinc 36 Br Bromine 37 Ag Silver 38 Hg Mercury 39 Au Gold 40 I Iodine 1 - 2 Post-translational 3 Co-translational 4 Pre-translational 5 Chemical derivative 6 Artefact 7 N-linked glycosylation 8 O-linked glycosylation 9 Other glycosylation 10 Synth. pep. protect. gp. 11 Isotopic label 12 Non-standard residue 13 Multiple 14 Other 1 H Hydrogen 1.007825035 1.00794 2 H2 Deuterium 2.014101779 2.014101779 3 Li Lithium 7.016003 6.941 4 C Carbon 12 12.0107 5 C13 Carbon13 13.00335483 13.00335483 6 N Nitrogen 14.003074 14.0067 7 N15 Nitrogen15 15.00010897 15.00010897 8 O Oxygen 15.99491463 15.9994 9 O18 Oxygen18 17.9991603 17.9991603 10 F Fluorine 18.99840322 18.9984032 11 Na Sodium 22.9897677 22.98977 12 P Phosphorous 30.973762 30.973761 13 S Sulfur 31.9720707 32.065 14 Cl Chlorine 34.96885272 35.453 15 K Potassium 38.9637074 39.0983 16 Ca Calcium 39.9625906 40.078 17 Fe Iron 55.9349393 55.845 18 Ni Nickel 57.9353462 58.6934 19 Zn Zinc 63.9291448 65.409 20 Se Selenium 79.9165196 78.96 21 Br Bromine 78.9183361 79.904 22 Ag Silver 106.905092 107.8682 23 Hg Mercury 201.970617 200.59 24 Au Gold 196.966543 196.96655 25 I Iodine 126.904473 126.90447 1 1 C 10 2 1 H 15 3 1 N 2 4 1 O 2 5 1 S 1 6 2 C 13 7 2 H 18 8 2 N 3 9 2 O 2 10 2 S 1 11 2 H2 4 12 3 C 15 13 3 H 22 14 3 N 3 15 3 O 3 16 3 S 1 17 3 H2 4 18 4 C 18 19 4 H 27 20 4 N 4 21 4 O 4 22 4 S 1 23 4 H2 8 24 5 C 20 25 5 H 29 26 5 N 4 27 5 O 5 28 5 S 2 29 5 H2 8 30 6 C 22 31 6 H 29 32 6 N 4 33 6 O 5 34 6 S 2 35 6 H2 8 36 7 C 10 37 7 H 15 38 7 N 2 39 7 O 2 40 7 S 1 41 8 C 13 42 8 H 22 43 8 N 3 44 8 O 2 45 8 S 1 46 9 C 15 47 9 H 26 48 9 N 3 49 9 O 3 50 9 S 1 51 10 C 18 52 10 H 35 53 10 N 4 54 10 O 4 55 10 S 1 56 11 C 20 57 11 H 37 58 11 N 4 59 11 O 5 60 11 S 2 61 12 C 22 62 12 H 37 63 12 N 4 64 12 O 5 65 12 S 2 1 12 2 12 3 12 4 12 5 12 6 12 7 13 8 13 9 13 10 13 11 13 12 13 418 1 O 1 417 1 C 2 416 1 H 2 190 2 O -1 189 2 N 1 188 2 H 1 511 3 S 1 510 3 O 2 509 3 N 2 508 3 C 10 507 3 H 14 888 4 O 1 887 4 N 1 886 4 C 2 885 4 H 3 1146 5 O 1 1145 5 N 1 1144 5 C 1 1143 5 H 1 813 6 O 2 812 6 C 2 811 6 H 2 193 7 O 1 192 7 N -1 191 7 H -1 993 8 S 1 992 8 O 6 991 8 N 4 990 8 C 22 989 8 H 38 999 9 S 1 998 9 O 6 997 9 N 4 996 9 C 22 995 9 H2 8 994 9 H 30 170 10 S -1 169 10 O 1 168 10 C -1 167 10 H -2 400 11 S -1 399 11 C -1 398 11 H -4 2870 12 S 1 2869 12 O 5 2868 12 N 4 2867 12 C 20 2866 12 H2 8 2865 12 H 26 2864 13 S 1 2863 13 O 5 2862 13 N 4 2861 13 C 20 2860 13 H 34 407 14 C 1 406 14 H 2 225 15 O 1 224 15 C 2 223 15 H 2 210 16 O 1 209 16 C 1 1461 17 O 1 1460 17 N 1 1459 17 C 5 1458 17 H 9 205 18 O18 1 204 18 O -1 411 19 O 1 392 20 S 1 391 20 O 5 390 20 N 4 389 20 C 18 388 20 H 30 436 21 P 1 435 21 O 3 434 21 H 1 439 22 P 1 438 22 O 3 437 22 H 1 402 23 O -1 401 23 H -2 256 24 O 1 255 24 N 1 254 24 C 3 253 24 H 5 443 25 O 1 442 25 N 1 441 25 C 7 440 25 H 5 369 26 N -1 368 26 H -3 212 27 O -1 211 27 H -2 178 28 N -1 177 28 H -3 447 29 O 2 446 29 N 1 445 29 C 6 444 29 H 9 413 30 Na 1 412 30 H -1 271 31 N 1 270 31 C 7 269 31 H 7 219 32 O 2 405 33 O 1 404 33 N -1 403 33 H -1 409 34 C 1 408 34 H 2 751 35 O 1 1154 36 C 2 1153 36 H 4 1148 37 C 3 1147 37 H 6 426 38 O 2 425 38 C 1 429 39 S 1 428 39 C 1 427 39 H 2 2665 40 S 1 2664 40 O 3 448 41 Hex 1 455 42 S 2 454 42 O 1 453 42 C 8 452 42 H 12 456 43 HexNAc 1 458 44 C 15 457 44 H 24 461 45 O 1 460 45 C 14 459 45 H 26 471 46 P 1 470 46 O 5 469 46 N 1 468 46 C 8 467 46 H 8 474 47 O 1 473 47 C 16 472 47 H 30 476 48 C 20 475 48 H 32 487 49 S 1 486 49 P 1 485 49 O 6 484 49 N 2 483 49 C 11 482 49 H 20 503 50 P 2 502 50 O 15 501 50 N 9 500 50 C 27 499 50 H 31 506 51 O 5 505 51 C 51 504 51 H 96 1311 52 N 2 1310 52 C 1 1309 52 H 2 282 53 O 2 281 53 C 9 280 53 H 16 451 54 O 6 450 54 C 6 449 54 H 8 481 55 S 1 480 55 O 6 479 55 N 3 478 55 C 10 477 55 H 15 569 56 O 1 568 56 C 2 567 56 H2 3 566 56 H -1 572 57 O 1 571 57 C 2 570 57 H 2 575 58 O 1 574 58 C 3 573 58 H 4 578 59 O 1 577 59 C13 3 576 59 H 4 619 60 O 1 618 60 N 1 617 60 C 7 616 60 H 13 615 61 O 1 614 61 N 1 613 61 C 7 612 61 H2 3 611 61 H 10 624 62 O 1 623 62 N 1 622 62 C 7 621 62 H2 6 620 62 H 7 629 63 O 1 628 63 N 1 627 63 C 7 626 63 H2 9 625 63 H 4 600 64 O 3 599 64 C 4 598 64 H 4 606 65 O 3 605 65 C 4 604 65 H2 4 603 66 O 3 602 66 C13 4 601 66 H 4 3472 358 O 1 3471 358 C 1 3460 355 N -2 3459 355 C -2 3457 354 O 2 2671 281 S 1 3438 348 O 1 3454 353 S 1 3453 353 O 2 3464 356 O 1 3458 355 H -6 3437 348 N -1 3442 349 O 2 3441 349 N -2 3452 353 N 3 3449 352 O 1 3446 351 O 1 3440 349 C -2 3439 349 H -2 2355 260 S 1 2354 260 P 1 2353 260 O 2 2352 260 H 1 2499 259 N15 2 2498 259 N -2 2497 259 C13 6 3456 354 N 1 3431 344 O 1 3430 344 N -3 3429 344 C -1 3428 344 H -5 3436 348 C -2 3435 348 H -1 790 89 S 1 789 89 O 1 788 89 N 3 787 89 C 10 786 89 H 15 795 90 S 1 794 90 O 2 793 90 N 4 792 90 C 16 791 90 H 26 801 91 S 1 800 91 O 2 799 91 N 4 798 91 C 16 797 91 H2 10 796 91 H 16 823 92 S 1 822 92 O 3 821 92 N 3 820 92 C 16 819 92 H 25 833 93 S 3 832 93 O 6 831 93 N 5 830 93 C 25 829 93 H 39 2454 94 N 2 2453 94 C 3 2452 94 H 4 2451 95 N 2 2450 95 C 3 2449 95 H2 4 3455 354 H -1 3451 353 C 10 3448 352 N -1 3447 352 H -3 859 97 O 1 858 97 N 1 857 97 C 3 856 97 H2 3 855 97 H 2 3450 353 H 15 3463 356 N -2 3462 356 C -3 969 105 O 3 2496 259 C -6 2347 258 O18 1 3445 351 C -1 3444 350 O 2 3443 350 C -1 3469 357 S 1 3468 357 O 2 3461 356 H -8 968 105 N 3 967 105 C 10 966 105 H 17 988 106 O 3 987 106 N 3 986 106 C13 9 985 106 C 1 984 106 H 17 1017 107 S 1 1016 107 O 2 1015 107 N 1 1014 107 C 6 1013 107 H 10 983 108 O 2 982 108 N 1 981 108 C 6 980 108 H 7 1111 112 S 1 1110 112 O 3 1109 112 N 4 2011 215 O 1 2010 215 N -1 1132 119 P 1 1131 119 C 22 1130 119 H 21 1108 112 C 16 1107 112 H 26 1106 113 S 1 1105 113 O 3 1104 113 N 4 1103 113 C 16 1102 113 H 24 1121 114 S 1 1120 114 O 3 1119 114 N 5 1118 114 C 16 1117 114 H 29 1116 115 S 1 1115 115 O 3 1114 115 N 5 1113 115 C 16 1112 115 H 27 1096 116 S 1 1095 116 O 3 1094 116 N 2 1093 116 C 15 1092 116 H 22 1101 117 S 1 1100 117 O 3 1099 117 N 2 1098 117 C 15 1097 117 H 24 1174 121 O 2 1142 118 S 3 1141 118 O 4 1140 118 N 4 1139 118 C 20 1138 118 H 34 1173 121 N 2 1172 121 C 4 1171 121 H 6 1425 122 O 1 1424 122 C 1 1195 123 Cl 1 1194 123 O 6 1193 123 N 1 1192 123 C 15 1191 123 H 20 1201 124 Cl 1 1200 124 O 6 1199 124 N 1 1198 124 C13 6 1197 124 C 9 1196 124 H 20 1205 125 C 2 1204 125 H2 4 1217 126 S 1 1216 126 O 1 1215 126 C 3 1214 126 H 4 1221 127 F 1 1220 127 H -1 1253 128 O 6 1252 128 N 1 1251 128 C 22 1250 128 H 14 1255 129 I 1 1254 129 H -1 1257 130 I 2 1256 130 H -2 1259 131 I 3 1258 131 H -3 1285 134 O 1 3470 358 H 2 3467 357 N 4 3466 357 C 10 3465 357 H 18 1284 134 C 14 1283 134 H 24 1288 135 O 1 1287 135 C 14 1286 135 H 22 1294 136 O 1 1293 136 C 7 1292 136 H 4 1423 162 Se 1 1422 162 S -1 1397 137 HexNAc 2 1396 137 Hex 5 1321 139 S 1 1320 139 O 2 1319 139 N 1 1318 139 C 12 1317 139 H 11 1333 140 O -1 1332 140 C -1 1331 140 H -1 1339 141 N 1 1338 141 C 2 1337 141 H 3 1354 142 HexNAc 1 1353 142 dHex 1 1342 143 HexNAc 2 1343 144 Hex 3 1344 145 dHex 2 1345 145 HexNAc 1 1346 146 dHex 1 1347 146 Hex 1 1348 146 HexNAc 1 1356 147 HexNAc 2 1355 147 dHex 1 1357 148 Hex 1 1358 148 HexNAc 2 1359 149 Hex 1 1360 149 HexNAc 1 1361 149 NeuAc 1 1362 150 dHex 2 1363 150 HexNAc 2 1364 151 Pent 1 1365 151 Hex 1 1366 151 HexNAc 2 1367 152 dHex 1 1368 152 Hex 1 1369 152 HexNAc 2 1370 153 Hex 2 1371 153 HexNAc 2 1372 154 Pent 1 1373 154 Hex 3 1374 154 HexNAc 1 1375 155 Pent 1 1376 155 dHex 1 1377 155 Hex 1 1378 155 HexNAc 2 1379 156 dHex 2 1380 156 Hex 1 1381 156 HexNAc 2 1382 157 Pent 1 1383 157 Hex 2 1384 157 HexNAc 2 1385 158 dHex 1 1386 158 Hex 2 1387 158 HexNAc 2 1399 159 HexNAc 2 1398 159 Hex 3 1390 160 Hex 1 1391 160 HexNAc 1 1392 160 NeuAc 2 1393 161 P 1 1394 161 Hex 3 1395 161 HexNAc 2 1492 171 S 1 1491 171 O 2 1490 171 N 1 1489 171 C13 6 1488 171 H 3 1472 170 O18 1 1471 170 O -1 1470 170 H 1 3187 330 C13 2 3154 327 S 1 3153 327 O -1 3152 327 C 2 3151 327 H 4 3142 298 C 1 1487 172 S 1 1486 172 O 2 1485 172 N 1 1484 172 C 6 1483 172 H 3 1758 194 O 1 1757 194 N 2 1756 194 C 10 1755 194 H 6 1720 189 C 2 1719 189 H2 6 1718 189 H -2 1578 176 O 1 1577 176 C 15 1576 176 H 22 1598 178 S 1 1597 178 O -1 1596 178 N 1 1595 178 C 4 1594 178 H 9 1588 179 O -1 1587 180 O -1 1586 180 C -1 1585 180 H -2 1593 181 O -1 1592 181 C 1 1591 181 H 1 1590 182 O -1 1589 182 H -1 3309 188 C13 6 3308 188 C -6 2670 281 P 3 2501 184 C13 9 2500 184 C -9 1712 185 P 1 1711 185 O 3 1710 185 C13 9 1709 185 C -9 1708 185 H 1 1648 186 O 2 1647 186 C 8 1646 186 H 4 1651 187 O 5 1650 187 C 16 1649 187 H 10 1728 193 O18 2 1727 193 O -2 2685 195 O 1 2684 195 N 2 2683 195 C 9 2682 195 H 18 2690 196 O 1 2689 196 N 2 2688 196 C 9 2687 196 H2 3 2686 196 H 16 2694 197 O 1 2693 197 N 2 2692 197 C 10 2691 197 H 20 2699 198 O 1 2698 198 N 2 2697 198 C 10 2696 198 H2 5 2695 198 H 15 1867 199 C 2 1866 199 H2 2 1865 199 H 2 1871 200 S 2 1870 200 O -1 1869 200 C 2 1868 200 H 4 2527 205 O 1 2326 211 O 1 2325 211 N 1 1860 202 O 1 1859 202 N 2 1858 202 C 9 1857 202 H 18 2703 203 O 1 2702 203 N 2 2701 203 C 9 2700 203 H 18 2526 205 C 6 2525 205 H 6 2522 206 O 1 2521 206 C 3 2520 206 H 4 2519 207 C 3 2518 207 H 2 2524 208 C 6 2523 208 H 4 2530 209 O 2 2529 209 C 6 2528 209 H 8 2324 211 C 4 2323 211 H 7 2331 212 O 1 2330 212 N 1 2329 212 C 4 2328 212 H2 5 2327 212 H 2 1942 213 P 2 1941 213 O 13 1940 213 N 5 1939 213 C 15 1938 213 H 21 1996 214 O 1 1995 214 N15 1 1994 214 N 1 1993 214 C13 3 1992 214 C 4 1991 214 H 12 2009 215 H -1 2346 258 O -1 2538 257 C 3 2537 257 H 6 3433 346 O 2 3432 345 O 3 3427 343 S 1 3426 343 O 2 3425 343 N 1 3424 343 C 9 3422 342 N 1 3343 340 H -1 3344 340 Br 1 3420 341 H -2 3421 342 H 1 2467 264 S 1 2517 268 N15 1 2516 268 N -1 2515 268 C13 5 2514 268 C -5 2462 262 H2 3 2461 262 H -3 2410 261 S 2 2409 261 O 3 2408 261 N 1 2407 261 C 7 2406 261 H 5 2536 256 C 3 2535 256 H 4 2534 255 C 2 2533 255 H 4 2313 243 Br 1 2312 243 O 2 2295 253 O 1 2294 253 C 4 2293 253 H 6 2532 254 C 2 2531 254 H 2 2311 243 N 2 2310 243 C 12 2309 243 H 13 2466 264 O -1 2465 264 N 1 2464 264 C 7 2463 264 H 7 2669 281 O 16 2668 281 N 7 2650 270 O 7 2649 270 C 19 2648 270 H 22 2653 271 O 8 2652 271 C 19 2651 271 H 24 2505 267 N15 4 2504 267 N -4 2503 267 C13 6 2502 267 C -6 2509 269 N15 1 2508 269 N -1 2507 269 C13 9 2506 269 C -9 2586 272 S 1 2585 272 O 4 2584 272 C 3 2583 272 H 4 2594 273 O 5 2593 273 N 1 2592 273 C 12 2591 273 H 15 2620 274 O 1 2619 274 C 1 2618 274 H 1 2601 275 O 1 2600 275 N 1 2614 276 S 1 2613 276 O 2 2612 276 N 1 2611 276 C 8 2610 276 H 9 2634 277 S 1 2633 277 C 1 2632 277 H 2 2631 278 O 1 2630 278 C 2 2629 278 H 4 2628 279 O 1 2655 280 C 2 2654 280 H 4 3434 347 O 2 2667 281 C 21 2666 281 H 34 2677 282 C 1 2676 282 H 2 2679 283 C 2 2678 283 H 4 2705 284 C 1 2704 284 H2 2 3217 285 S 1 3216 285 O 2 3215 285 N 1 3214 285 C 6 3213 285 H 5 3207 286 S 1 3206 286 O 2 3205 286 N 1 3204 286 C13 6 3203 286 H 5 2768 289 S 1 2745 288 O 1 2744 288 H -2 2767 289 O 3 2766 289 N 4 2765 289 C 16 2764 289 H 28 2773 290 S 2 2772 290 O 3 2771 290 N 4 2770 290 C 19 2769 290 H 32 2779 292 P 1 2774 291 Hg 1 2778 292 O 9 2777 292 N 2 2776 292 C 9 2775 292 H 11 2794 293 S 1 2793 293 O 2 2792 293 N 1 2791 293 C 5 2790 293 H 7 2799 294 S 1 2798 294 O 3 2797 294 N 4 2796 294 C 14 2795 294 H 22 2835 295 O 4 2834 295 C 6 2833 295 H 10 2840 296 S 2 2839 296 O 3 2838 296 N 1 2837 296 C 7 2836 296 H 5 2845 297 S 2 2844 297 O 3 2843 297 N 3 2842 297 C 8 2841 297 H 7 3141 298 H2 3 3140 298 H -1 2856 299 O 2 2855 299 C 1 2859 300 O 2 2858 300 C 2 2857 300 H 2 2909 301 O 2 2908 301 N 2 2907 301 C 10 2906 301 H 10 2905 302 O 2 2904 302 C 11 2903 302 H 6 2924 303 S 1 2923 303 O 1 2922 303 C 2 2921 303 H 4 3101 319 O 1 3023 305 HexNAc 4 3022 305 Hex 3 3021 305 dHex 1 3009 306 S 1 3008 306 O 3 3073 317 O -1 3031 307 HexNAc 4 3030 307 Hex 4 3029 307 dHex 1 3045 316 C 6 3036 308 HexNAc 4 3035 308 Hex 5 3034 308 dHex 1 3100 319 C 3 3020 309 HexNAc 4 3019 309 Hex 3 3103 318 C 5 3102 318 H 2 3028 310 HexNAc 4 3027 310 Hex 4 3072 317 H -2 3044 316 H 6 3033 311 HexNAc 4 3032 311 Hex 5 3018 312 S 1 3017 312 O 2 3016 312 N 1 3015 312 C 3 3014 312 H 6 3007 313 O -1 3006 313 N -2 3005 313 C -6 3004 313 H -12 3013 314 O 2 3012 314 N 1 3011 314 C 5 3010 314 H 5 3041 315 S 1 3040 315 O 5 3039 315 N 3 3038 315 C 21 3037 315 H 15 3099 319 H 2 3109 320 O 3 3108 320 N 1 3107 320 C 6 3106 320 H 9 3105 321 O -1 3104 321 H -1 3186 330 H2 6 3184 329 C13 1 3183 329 H2 3 3182 329 H -1 3115 323 I 1 3114 323 S 1 3113 323 O 6 3112 323 N 4 3111 323 C 31 3110 323 H 30 3126 324 Cl 1 3125 324 S 1 3124 324 N 1 3123 324 C 3 3122 324 H 6 3356 325 S 1 3355 325 P 1 3354 325 O 5 3353 325 N 4 3352 325 C 27 3351 325 H 50 3185 330 H -2 3304 334 S 1 3303 334 O 2 3302 334 N 1 3301 334 C 5 3259 332 S 3 3258 332 P 1 3257 332 O 5 3256 332 N 4 3255 332 C 19 3254 332 H 34 3423 343 H 13 3350 333 S 1 3349 333 P 1 3348 333 O 5 3347 333 N 3 3346 333 C 19 3345 333 H 35 3300 334 H 8 3307 335 O 2 3306 335 C 9 3305 335 H 18 3477 360 O -1 3476 360 C -1 3474 359 O 1 3473 359 H -2 3299 337 O -1 3298 337 N 1 3297 337 C 1 3296 337 H 3 3482 361 S 1 3481 361 O 1 3475 360 H -2 3480 361 N 4 3479 361 C 10 3478 361 H 16 1 Acetylation Acetyl 42.010565 42.0367 H(2) C(2) O unimod admin 2002-08-19 19:17:11 2002-10-04 15:44:08 2 Amidation Amide -0.984016 -0.9848 H N O(-1) unimod admin 2002-08-19 19:17:11 2002-10-01 12:24:24 3 Biotinylation Biotin 226.077598 226.2954 H(14) C(10) N(2) O(2) S unimod admin 2002-08-19 19:17:11 2002-10-04 19:23:43 4 Iodoacetamide derivative Carbamidomethyl 57.021464 57.0513 H(3) C(2) N O unimod admin 2002-08-19 19:17:11 2003-01-27 08:52:34 5 Carbamylation Carbamyl 43.005814 43.0247 H C N O unimod admin 2002-08-19 19:17:11 2003-04-02 17:01:34 6 Iodoacetic acid derivative Carboxymethyl 58.005479 58.0361 H(2) C(2) O(2) unimod admin 2002-08-19 19:17:11 2002-12-02 17:49:27 7 Deamidation Deamidation 0.984016 0.9848 H(-1) N(-1) O unimod admin 2002-08-19 19:17:11 2002-10-01 12:27:16 8 Gygi ICAT(TM) d0 Gygi_ICATd0 486.251206 486.6253 H(38) C(22) N(4) O(6) S unimod admin 2002-08-19 19:17:11 2003-02-01 18:37:27 9 Gygi ICAT(TM) d8 Gygi_ICATd8 494.301420 494.6746 H(30) H2(8) C(22) N(4) O(6) S unimod admin 2002-08-19 19:17:11 2003-02-01 18:38:02 10 Homoserine HSe -29.992806 -30.0922 H(-2) C(-1) O S(-1) Cyanogen bromide (CNBr) cleavage converts the C-term Met to either homoserine or homoserine lactone, depending on pH. unimod admin 2002-08-19 19:17:11 2002-10-01 11:31:37 11 Homoserine lactone Hse_lact -48.003371 -48.1075 H(-4) C(-1) S(-1) Cyanogen bromide (CNBr) cleavage converts the C-term Met to either homoserine or homoserine lactone, depending on pH. unimod admin 2002-08-19 19:17:11 2002-10-04 15:21:02 12 Applied Biosystems original ICAT(TM) d8 AB_old_ICATd8 450.275205 450.6221 H(26) H2(8) C(20) N(4) O(5) S unimod admin 2002-08-19 19:17:11 2005-04-04 13:25:50 13 Applied Biosystems original ICAT(TM) d0 AB_old_ICATd0 442.224991 442.5728 H(34) C(20) N(4) O(5) S unimod admin 2002-08-19 19:17:11 2005-04-04 13:25:36 14 Methyl ester Me-ester 14.015650 14.0266 H(2) C unimod admin 2002-08-19 19:17:11 2002-10-04 15:33:34 15 N-Acetylation N-Acetyl 42.010565 42.0367 H(2) C(2) O unimod admin 2002-08-19 19:17:11 2002-10-01 14:12:45 16 N-Formylation N-Formyl 27.994915 28.0101 C O unimod admin 2002-08-19 19:17:11 2002-10-01 13:08:44 17 N-isopropylcarboxamidomethyl NIPCAM 99.068414 99.1311 H(9) C(5) N O unimod admin 2002-08-19 19:17:11 2003-11-24 11:56:39 18 O18 label O18 2.004246 1.9998 O(-1) O18 unimod admin 2002-08-19 19:17:11 2002-10-01 12:55:56 19 Oxidation Oxidation 15.994915 15.9994 O unimod admin 2002-08-19 19:17:11 2002-10-04 15:39:31 20 Biotinyl-iodoacetamidyl-3,6-dioxaoctanediamine PEO-Biotin 414.193691 414.5196 H(30) C(18) N(4) O(5) S unimod admin 2002-08-19 19:17:11 2002-10-03 08:51:45 21 Phosphorylation Phospho 79.966331 79.9799 H O(3) P unimod admin 2002-08-19 19:17:11 2002-10-04 15:53:04 22 Phosphorylation without neutral loss Phospho-NL 79.966331 79.9799 H O(3) P unimod admin 2002-08-19 19:17:11 2002-10-04 15:54:20 23 Phosphorylation with prompt loss of phosphate Phospho+PL -18.010565 -18.0153 H(-2) O(-1) Unusual under normal conditions unimod admin 2002-08-19 19:17:11 2002-10-04 15:28:12 24 Acrylamide adduct Propionamide 71.037114 71.0779 H(5) C(3) N O unimod admin 2002-08-19 19:17:11 2002-10-01 15:02:32 25 Pyridyl Pyridyl 119.037114 119.1207 H(5) C(7) N O unimod admin 2002-08-19 19:17:11 2002-10-04 15:55:58 26 S-carbamoylmethylcysteine cyclization (N-terminus) Pyro-cmC -17.026549 -17.0305 H(-3) N(-1) Cyclisation of N-term carbamidomethyl-Cys unimod admin 2002-08-19 19:17:11 2002-10-01 18:20:18 27 Pyro-glu from E Pyro-glu -18.010565 -18.0153 H(-2) O(-1) unimod admin 2002-08-19 19:17:11 2002-10-01 14:00:43 28 Pyro-glu from Q Pyro-glu -17.026549 -17.0305 H(-3) N(-1) unimod admin 2002-08-19 19:17:11 2002-10-01 11:54:07 29 N-Succinimidyl-3-morpholine acetate SMA 127.063329 127.1412 H(9) C(6) N O(2) unimod admin 2002-08-19 19:17:11 2002-10-04 15:57:39 30 Sodium adduct Sodiated 21.981943 21.9818 H(-1) Na Proton replaced by sodium cation unimod admin 2002-08-19 19:17:11 2002-10-04 15:40:29 31 S-pyridylethylation S-pyridylethyl 105.057849 105.1372 H(7) C(7) N unimod admin 2002-08-19 19:17:11 2002-10-01 15:07:12 32 Sulphone Sulphone 31.989829 31.9988 O(2) unimod admin 2002-08-19 19:17:11 2002-10-01 14:11:18 33 Citrullination Citrullination 0.984016 0.9848 H(-1) N(-1) O unimod admin 2002-08-19 19:17:11 2002-10-04 15:31:54 34 Methylation Methyl 14.015650 14.0266 H(2) C unimod admin 2002-08-19 19:17:11 2002-10-04 15:34:54 35 Hydroxylation Hydroxylation 15.994915 15.9994 O unimod admin 2002-08-19 19:17:11 2002-11-24 13:22:35 36 di-Methylation di-Methylation 28.031300 28.0532 H(4) C(2) unimod admin 2002-08-19 19:17:11 2003-04-23 08:12:11 37 tri-Methylation tri-Methylation 42.046950 42.0797 H(6) C(3) unimod admin 2002-08-19 19:17:11 2003-04-14 17:27:59 38 Gamma-carboxylation Gamma-carboxyl 43.989829 44.0095 C O(2) unimod admin 2002-08-19 19:17:11 2002-10-04 15:48:04 39 Beta-methylthiolation b-methylthiol 45.987721 46.0916 H(2) C S unimod admin 2002-08-19 19:17:11 2002-10-04 15:49:12 40 O-Sulfonation Sulfation 79.956815 80.0632 O(3) S unimod admin 2002-08-19 19:17:11 2005-02-05 12:05:52 41 Hexose Hex 162.052823 162.1406 Hex unimod admin 2002-08-19 19:17:11 2002-10-04 15:59:27 42 Lipoyl Lipoyl 188.032956 188.3103 H(12) C(8) O S(2) unimod admin 2002-08-19 19:17:11 2002-10-04 16:01:24 43 N-Acetylhexosamine HexNAc 203.079373 203.1925 HexNAc unimod admin 2002-08-19 19:17:11 2002-10-04 16:02:17 44 Farnesylation Farnesylation 204.187801 204.3511 H(24) C(15) unimod admin 2002-08-19 19:17:11 2002-10-04 16:04:02 45 Myristoylation Myristoylation 210.198366 210.3556 H(26) C(14) O unimod admin 2002-08-19 19:17:11 2002-10-04 16:05:05 46 Pyridoxal phosphate Pyridoxal-phos 229.014009 229.1266 H(8) C(8) N O(5) P unimod admin 2002-08-19 19:17:11 2002-10-04 16:06:37 47 Palmitoylation Palmitoylation 238.229666 238.4088 H(30) C(16) O unimod admin 2002-08-19 19:17:11 2002-10-04 16:07:44 48 Geranyl-geranyl Geranyl-geranyl 272.250401 272.4681 H(32) C(20) unimod admin 2002-08-19 19:17:11 2002-10-04 16:08:55 49 Phosphopantetheine p-pantetheine 339.077969 339.3251 H(20) C(11) N(2) O(6) P S unimod admin 2002-08-19 19:17:11 2002-10-04 16:10:34 50 Flavin adenine dinucleotide FAD 783.141486 783.5339 H(31) C(27) N(9) O(15) P(2) unimod admin 2002-08-19 19:17:11 2002-10-04 16:13:08 51 N-acyl diglyceride cysteine Tripalmitate 788.725777 789.3049 H(96) C(51) O(5) unimod admin 2002-08-19 19:17:11 2002-10-04 16:13:49 52 Guanidination Guanidination 42.021798 42.0400 H(2) C N(2) Specific for sidechain of lysine. Does not modify the N-termini except for glycine at a slower rate than the side chain of lysine. unimod admin 2002-08-19 19:17:11 2003-08-16 11:36:45 53 4-hydroxynonenal (HNE) HNE 156.115030 156.2221 H(16) C(9) O(2) A lipid-type modification. HNE forms a Michael addition product on Cysteine, Histidine and Lysines. Unusually, it doesn't replace a hydrogen on the amino acid side chain. unimod admin 2002-08-19 19:17:11 2002-10-01 15:48:40 54 N-glucuronylation glucuronyl 176.032088 176.1241 H(8) C(6) O(6) jcottrell users 2002-10-01 21:36:48 2002-10-04 16:00:32 55 glutathione disulfide Glutathione 305.068156 305.3076 H(15) C(10) N(3) O(6) S unimod admin 2002-10-03 09:10:19 2002-10-04 16:09:43 56 Acetate labeling reagent (N-term & K) (heavy form, +3amu) Acetyl_heavy 45.029395 45.0552 H(-1) H2(3) C(2) O penner users 2002-10-16 16:41:56 2002-10-20 10:49:18 57 Acetate labeling reagent light form (N-term & K) Acetyl_light 42.010565 42.0367 H(2) C(2) O penner users 2002-10-16 16:49:47 2002-10-20 10:50:36 58 Propionate labeling reagent light form (N-term & K) Propionyl_light 56.026215 56.0633 H(4) C(3) O penner users 2002-10-16 16:52:38 2002-10-20 10:51:31 59 Propionate labeling reagent heavy form (+3amu), N-term&K Propionyl_heavy 59.036279 59.0412 H(4) C13(3) O penner users 2002-10-16 16:55:47 2002-10-20 10:52:26 60 Quaternary amine labeling reagent light form (N-term & K) Quat_0 127.099714 127.1842 H(13) C(7) N O penner users 2002-10-16 17:02:37 2002-11-07 15:58:35 61 Quaternary amine labeling reagent heavy (+3amu) form, N-term & K Quat_3 130.118544 130.2027 H(10) H2(3) C(7) N O penner users 2002-10-16 17:09:34 2002-11-07 15:57:50 62 Quaternary amine labeling reagent heavy form (+6amu), N-term & K Quat_6 133.137375 133.2212 H(7) H2(6) C(7) N O penner users 2002-10-16 17:12:27 2002-11-07 15:59:46 63 Quaternary amine labeling reagent heavy form (+9amu), N-term & K Quat_9 136.156205 136.2397 H(4) H2(9) C(7) N O penner users 2002-10-16 17:14:22 2002-11-07 16:00:44 64 Succinic anhydride labeling reagent light form (N-term & K) Suc_anh_light 100.016044 100.0728 H(4) C(4) O(3) penner users 2002-10-16 17:17:07 2002-10-20 10:56:20 65 Succinic anhydride labeling reagent, heavy form (+4amu, 4H2), N-term & K Suc_anh+4H2 104.041151 104.0974 H2(4) C(4) O(3) penner users 2002-10-16 17:19:51 2002-10-20 10:57:50 66 Succinic anhydride labeling reagent, heavy form (+4amu, 4C13), N-term & K Suc_anh+4C13 104.029463 104.0434 H(4) C13(4) O(3) penner users 2002-10-16 17:23:58 2002-10-20 10:57:02 357 oxidized proline biotinylated with biotin hydrazide probiotinhydrazide 258.115047 258.3405 H(18) C(10) N(4) O(2) S unimod admin 2005-09-10 22:56:20 2005-09-10 23:11:29 358 Proline oxidation to glutamic semialdehyde Proglutamicsealde 30.010565 30.0260 H(2) C O unimod admin 2005-09-10 22:57:32 2005-09-10 23:11:50 359 proline oxidation to pyroglutamic acid Pyroglutamic 13.979265 13.9835 H(-2) O unimod admin 2005-09-10 22:58:42 2005-09-10 23:12:13 346 phenylalanine oxidation to dihydroxyphenylalanine dihydroxy-phe 31.989829 31.9988 O(2) unimod admin 2005-09-10 22:41:05 2005-09-10 23:04:46 347 tryptophan oxidation to formylkynurenin formylkynurenin 31.989829 31.9988 O(2) unimod admin 2005-09-10 22:42:13 2005-09-10 23:05:08 348 histidine oxidation to aspargine His2Asn -23.015984 -23.0366 H(-1) C(-2) N(-1) O unimod admin 2005-09-10 22:43:27 2005-09-10 23:06:52 349 histidine oxidation to aspartic acid His2Asp -22.031969 -22.0519 H(-2) C(-2) N(-2) O(2) unimod admin 2005-09-10 22:44:32 2005-09-10 23:07:15 350 tryptophan oxidation to hydroxykynurenin hydroxykynurenin 19.989829 19.9881 C(-1) O(2) unimod admin 2005-09-10 22:47:20 2005-09-10 23:07:35 256 Propionaldehyde +40 Propionald+40 40.031300 40.0639 H(4) C(3) fatcat users 2004-08-12 20:04:01 2004-11-06 17:00:21 255 Acetaldehyde +28 Acetald+28 28.031300 28.0532 H(4) C(2) fatcat users 2004-08-12 20:02:38 2004-11-06 16:59:58 356 glutamine oxidation to 2-oxo-propanoic acid (ethandioic acid) OxGln2 -56.073834 -56.1096 H(-8) C(-3) N(-2) O unimod admin 2005-09-10 22:55:01 2005-09-10 23:11:08 344 Arginine oxidation to glutamic semialdehyde Argglutamicsealde -43.053433 -43.0711 H(-5) C(-1) N(-3) O unimod admin 2005-09-10 22:38:24 2005-09-10 23:03:35 345 cysteine oxidation to cysteic acid Cysteic_acid 47.984744 47.9982 O(3) unimod admin 2005-09-10 22:39:38 2005-09-10 23:03:58 89 Iminobiotinylation Im_biotin 225.093583 225.3106 H(15) C(10) N(3) O S toppolzer users 2002-11-25 16:01:48 2002-12-01 12:51:20 90 ESP-Tag light d0 ESP-Tag_light 338.177647 338.4682 H(26) C(16) N(4) O(2) S toppolzer users 2002-11-25 16:12:50 2002-12-01 12:52:55 91 ESP-Tag heavy d10 ESP-Tag_heavy 348.240414 348.5299 H(16) H2(10) C(16) N(4) O(2) S toppolzer users 2002-11-25 16:18:58 2002-12-01 12:53:38 92 NHS-LC-Biotin Biot_LC 339.161662 339.4530 H(25) C(16) N(3) O(3) S toppolzer users 2002-12-04 09:55:19 2002-12-07 11:30:34 93 EDT-maleimide-PEO-biotin EDT-m-biotin 601.206246 601.8021 H(39) C(25) N(5) O(6) S(3) take users 2002-12-27 09:08:56 2003-01-05 11:37:15 94 IMID d0 IMID_light 68.037448 68.0773 H(4) C(3) N(2) Liao users 2003-01-09 04:14:06 2004-10-07 15:04:34 95 IMID d4 IMID_heavy 72.062555 72.1019 H2(4) C(3) N(2) Liao users 2003-01-09 04:15:25 2004-10-07 15:03:57 353 oxidized Lysine biotinylated with biotin hydrazide Lysbiotinhydrazide 241.088497 241.3100 H(15) C(10) N(3) O(2) S unimod admin 2005-09-10 22:51:13 2005-09-10 23:09:06 97 Acrylamide d3 Acrylamide_heavy 74.055944 74.0964 H(2) H2(3) C(3) N O Liao users 2003-01-14 08:10:30 2003-01-19 12:28:21 354 Oxidation to nitro Nitro 44.985078 44.9976 H(-1) N O(2) unimod admin 2005-09-10 22:52:38 2005-09-10 23:10:34 355 glutamine oxidation to 2-oxo-propanoic acid OxGln1 -58.053098 -58.0824 H(-6) C(-2) N(-2) unimod admin 2005-09-10 22:53:56 2005-09-10 23:10:55 105 Applied Biosystems cleavable ICAT(TM) light ICAT_light 227.126991 227.2603 H(17) C(10) N(3) O(3) tpjd2 users 2003-01-27 10:27:11 2003-02-01 17:39:53 254 Acetaldehyde +26 Acetald+26 26.015650 26.0373 H(2) C(2) fatcat users 2004-08-12 20:01:03 2004-11-06 16:59:35 351 tryptophan oxidation to kynurenin kynurenin 3.994915 3.9887 C(-1) O unimod admin 2005-09-10 22:48:33 2005-09-10 23:08:04 352 Lysine oxidation to aminoadipic semialdehyde Lysaminoadipicsealde -1.031634 -1.0311 H(-3) N(-1) O unimod admin 2005-09-10 22:49:59 2005-09-10 23:08:46 106 Applied Biosystems cleavable ICAT(TM) heavy ICAT_heavy 236.157185 236.1942 H(17) C C13(9) N(3) O(3) tpjd2 users 2003-01-27 10:32:53 2003-02-01 18:10:57 107 Addition of N-formyl met +N-formyl-met 160.043224 160.2141 H(10) C(6) N O(2) S jphilip users 2003-01-27 18:24:14 2003-02-16 17:30:25 108 N-ethylmaleimide on cysteines NEM 125.047679 125.1253 H(7) C(6) N O(2) Pflieger users 2003-01-30 09:52:51 2003-02-01 17:44:44 112 Oxidized lysine biotinylated with biotin-LC-hydrazide, reduced OxLysBiotinRed 354.172561 354.4676 H(26) C(16) N(4) O(3) S S_Lee users 2003-02-21 00:53:43 2003-02-27 17:49:14 215 glycosylated asparagine Glyc-Asn 0.984016 0.9848 H(-1) N(-1) O Convertion of glycosylated asparagine residues upon deglycosylation with PGNase F in H2O. fanxiaol users 2004-06-22 22:45:37 2004-06-25 18:13:40 119 Thio Ether Formation - BTP Adduct IBTP 316.138088 316.3759 H(21) C(22) P MRCDunn users 2003-03-13 09:36:40 2003-03-15 09:59:44 113 Oxidized lysine biotinylated with biotin-LC-hydrazide OxLysBiotin 352.156911 352.4518 H(24) C(16) N(4) O(3) S S_Lee users 2003-02-21 01:25:11 2003-02-27 17:48:44 114 Oxidized proline biotinylated with biotin-LC-hydrazide, reduced OxProBiotinRed 371.199111 371.4982 H(29) C(16) N(5) O(3) S S_Lee users 2003-02-21 01:34:28 2003-02-27 17:50:23 115 Oxidized Proline biotinylated with biotin-LC-hydrazide OxProBiotin 369.183461 369.4823 H(27) C(16) N(5) O(3) S S_Lee users 2003-02-21 01:35:44 2003-02-27 17:49:54 116 Oxidized arginine biotinylated with biotin-LC-hydrazide OxArgBiotin 310.135113 310.4118 H(22) C(15) N(2) O(3) S S_Lee users 2003-02-21 01:41:19 2003-02-27 17:47:47 117 Oxidized arginine biotinylated with biotin-LC-hydrazide, reduced OxArgBiotinRed 312.150763 312.4277 H(24) C(15) N(2) O(3) S S_Lee users 2003-02-21 01:43:00 2003-02-27 17:48:14 118 EDT-iodo-PEO-biotin EDT-i-biotin 490.174218 490.7034 H(34) C(20) N(4) O(4) S(3) take users 2003-02-24 07:18:42 2003-03-19 06:06:29 121 ubiquitinylation residue GlyGly 114.042927 114.1026 H(6) C(4) N(2) O(2) The two glycine residues left on ubiquitinylated lysine after tryptic digestion gielbert users 2003-04-30 13:32:37 2003-05-05 19:18:54 122 Formylation Formyl 27.994915 28.0101 C O pnacman users 2003-05-01 13:28:36 2003-10-20 14:40:48 123 N-iodoacetyl, p-chlorobenzyl-12C6-glucamine Harbury_ICAT_C12 345.097915 345.7754 H(20) C(15) N O(6) Cl allis users 2003-05-07 19:54:20 2003-05-10 11:42:31 124 N-iodoacetyl, p-chlorobenzyl-13C6-glucamine Harbury_ICAT_C13 351.118044 351.7313 H(20) C(9) C13(6) N O(6) Cl allis users 2003-05-07 19:56:12 2003-05-10 11:43:02 125 reductive amination-D RA-D 32.056407 32.0778 H2(4) C(2) PhilipHsu users 2003-05-19 10:27:16 2003-05-24 17:43:59 126 thioacylation of primary amines (N-term and Lys) DSP 87.998285 88.1283 H(4) C(3) O S rabah users 2003-06-05 13:38:19 2003-06-15 19:24:42 127 fluorophenylalanine replacement of phenylalanine Fphe 17.990578 17.9905 H(-1) F jschulte users 2003-06-19 19:54:25 2003-06-23 18:37:26 128 5-Iodoacetamidofluorescein (Molecular Probe, Eugene, OR) Fluorescein 388.082112 388.3497 H(14) C(22) N O(6) Philip users 2003-06-25 02:58:18 2003-07-16 11:54:23 129 Iodination Iodination 125.896648 125.8965 H(-1) I brettsp users 2003-07-10 15:43:17 2003-07-16 11:56:57 130 di-Iodination di-Iodination 251.793296 251.7931 H(-2) I(2) brettsp users 2003-07-10 15:49:42 2003-07-16 11:58:17 131 tri-Iodination tri-Iodination 377.689944 377.6896 H(-3) I(3) brettsp users 2003-07-10 15:51:32 2003-07-16 11:59:04 134 (cis-delta 5)-tetradecaenoyl myristoleylation 208.182715 208.3398 H(24) C(14) O Found on vision signal transduction proteins tomneubert users 2003-07-18 21:30:51 2003-07-21 19:06:01 360 Proline oxidation to pyrrolidinone Pyrrolidinone -30.010565 -30.0260 H(-2) C(-1) O(-1) unimod admin 2005-09-10 23:00:19 2005-09-10 23:12:33 135 (cis,cis-delta 5, delta 8)-tetradecadienoyl myristoyl-4H 206.167065 206.3239 H(22) C(14) O Found on vision signal transduction proteins tomneubert users 2003-07-18 21:36:44 2003-07-21 19:09:29 136 labeling reagent light form (N-term & K) benzoyl 104.026215 104.1061 H(4) C(7) O samirjulka users 2003-07-31 22:24:05 2003-08-05 08:58:37 137 N-linked glycan core N-glycan 1216.422863 1217.0880 Hex(5) HexNAc(2) tpjd2 users 2003-08-06 11:31:37 2003-09-29 13:12:46 139 5-dimethylaminonaphthalene-1-sulfonyl dansyl 233.051049 233.2862 H(11) C(12) N O(2) S allis users 2003-08-19 02:51:24 2003-08-24 18:31:18 140 ISD a-series (C-Term) a-type_Ion -29.002740 -29.0180 H(-1) C(-1) O(-1) MS/MS experiments of mass spectrometric a-ions (MS^3) can be used for protein identification by library searching. T3-sequencing is such a technique (see reference). Search engines must recognize this ''virtual modification'' for this purpose. suckau users 2003-09-02 16:17:09 2003-09-06 20:11:50 141 amidination of lysines or N-terminal amines with methyl acetimidate amidine 41.026549 41.0519 H(3) C(2) N pnacman users 2003-09-25 11:04:41 2003-09-27 18:50:59 142 HexNAc1dHex1 HexNAc1dHex1 349.137281 349.3337 dHex HexNAc unimod admin 2003-09-29 12:28:45 2003-09-29 12:46:06 143 HexNAc2 HexNAc2 406.158745 406.3850 HexNAc(2) unimod admin 2003-09-29 12:37:55 2003-09-29 12:37:55 144 Hex3 Hex3 486.158471 486.4218 Hex(3) unimod admin 2003-09-29 12:39:30 2003-09-29 12:39:30 145 HexNAc1dHex2 HexNAc1dHex2 495.195190 495.4749 dHex(2) HexNAc unimod admin 2003-09-29 12:40:57 2003-09-29 12:40:57 146 Hex1HexNAc1dHex1 Hex1HexNAc1dHex1 511.190105 511.4743 dHex Hex HexNAc unimod admin 2003-09-29 12:42:27 2003-09-29 12:42:27 147 HexNAc2dHex1 HexNAc2dHex1 552.216654 552.5262 dHex HexNAc(2) unimod admin 2003-09-29 12:44:18 2003-09-29 12:46:32 148 Hex1HexNAc2 Hex1HexNAc2 568.211569 568.5256 Hex HexNAc(2) unimod admin 2003-09-29 12:48:18 2003-09-29 12:48:18 149 Hex1HexNAc1NeuAc1 Hex1HexNAc1NeuAc1 656.227613 656.5877 Hex HexNAc NeuAc unimod admin 2003-09-29 12:51:19 2003-09-29 12:51:19 150 HexNAc2dHex2 HexNAc2dHex2 698.274563 698.6674 dHex(2) HexNAc(2) unimod admin 2003-09-29 12:52:36 2003-09-29 12:52:36 151 Hex1HexNAc2Pent1 Hex1HexNAc2Pent1 700.253828 700.6403 Pent Hex HexNAc(2) unimod admin 2003-09-29 12:53:29 2003-09-29 12:53:29 152 Hex1HexNAc2dHex1 Hex1HexNAc2dHex1 714.269478 714.6668 dHex Hex HexNAc(2) unimod admin 2003-09-29 12:54:18 2003-09-29 12:54:18 153 Hex2HexNAc2 Hex2HexNAc2 730.264392 730.6662 Hex(2) HexNAc(2) unimod admin 2003-09-29 12:55:08 2003-09-29 12:55:07 154 Hex3HexNAc1Pent1 Hex3HexNAc1Pent1 821.280102 821.7289 Pent Hex(3) HexNAc unimod admin 2003-09-29 12:56:02 2003-09-29 12:56:01 155 Hex1HexNAc2dHex1Pent1 Hex1HexNAc2dHex1Pent1 846.311736 846.7815 Pent dHex Hex HexNAc(2) unimod admin 2003-09-29 12:57:01 2003-09-29 12:57:01 156 Hex1HexNAc2dHex2 Hex1HexNAc2dHex2 860.327386 860.8080 dHex(2) Hex HexNAc(2) unimod admin 2003-09-29 12:57:52 2003-09-29 12:57:52 157 Hex2HexNAc2Pent1 Hex2HexNAc2Pent1 862.306651 862.7809 Pent Hex(2) HexNAc(2) unimod admin 2003-09-29 12:58:38 2003-09-29 12:58:38 158 Hex2HexNAc2dHex1 Hex2HexNAc2dHex1 876.322301 876.8074 dHex Hex(2) HexNAc(2) unimod admin 2003-09-29 12:59:18 2003-09-29 12:59:18 159 Hex3HexNAc2 Hex3HexNAc2 892.317216 892.8068 Hex(3) HexNAc(2) unimod admin 2003-09-29 12:59:59 2003-09-29 13:14:27 160 Hex1HexNAc1NeuAc2 Hex1HexNAc1NeuAc2 947.323029 947.8423 Hex HexNAc NeuAc(2) unimod admin 2003-09-29 13:01:06 2003-09-29 13:01:05 161 Hex3HexNAc2P1 Hex3HexNAc2P1 923.290978 923.7806 P Hex(3) HexNAc(2) unimod admin 2003-09-29 13:02:02 2003-09-29 13:02:02 162 Selenium replaces sulphur in Methionine SeMet 47.944449 46.8950 S(-1) Se pnacman users 2003-10-14 11:24:28 2003-10-20 14:33:37 171 Shimadzu 13CNBS 13CNBS 159.008578 159.1144 H(3) C13(6) N O(2) S kuyama users 2004-01-07 07:22:34 2004-01-11 10:19:54 329 monomethylated arginine Methyl-Heavy 18.037835 18.0377 H(-1) H2(3) C13 sams users 2005-06-30 18:19:34 2005-07-09 17:26:12 330 dimethylated arginine Dimethyl-Heavy 36.075670 36.0754 H(-2) H2(6) C13(2) sams users 2005-06-30 18:21:32 2005-07-09 17:26:44 172 Shimadzu 12CNBS 12CNBS 152.988449 153.1585 H(3) C(6) N O(2) S kuyama users 2004-01-07 07:25:07 2004-01-11 10:19:23 170 glycosylated asparagine 18O labeling GN18Olabeling 3.012071 3.0077 H O(-1) O18 This modification accounts for convertion of glycosylated asparagine residues upon deglycosylation with PGNase F in H218O. lobvi users 2003-12-11 18:24:58 2003-12-15 19:29:09 195 APTA-d0 QAT 170.141913 170.2520 H(18) C(9) N(2) O s_julka users 2004-04-08 17:12:14 2005-02-14 11:55:26 176 Michael addition of BHT quinone methide to Cysteine and Lysine BHT 218.167065 218.3346 H(22) C(15) O BHT metabolism has been studied in rats and mice in relation to tumor promotion. gonzo users 2004-02-05 22:02:53 2004-02-14 22:17:22 327 S-Ethylcystine from Serine S-Eth 44.008456 44.1188 H(4) C(2) O(-1) S Phosphoserine to S-ethylcystine via Beta elimination/Michael addition of ethylthiol UCDMSF users 2005-06-20 20:09:02 2005-06-25 08:55:48 178 phosphorylation to amine thiol ser_thr_DAET 87.050655 87.1866 H(9) C(4) N O(-1) S DAET = 2-(dimethylamino)ethanethiol chemgirl18 users 2004-02-11 20:34:16 2004-02-15 17:54:33 179 Serine to Alanine ser_ala -15.994915 -15.9994 O(-1) mdurham users 2004-02-12 20:19:24 2004-02-14 22:24:24 180 Threonine to Alanine thr_ala -30.010565 -30.0260 H(-2) C(-1) O(-1) mdurham users 2004-02-12 20:20:35 2004-02-14 22:23:58 181 Serine reduced to aminobutynate ser_ambut -2.987090 -2.9808 H C O(-1) mdurham users 2004-02-12 20:41:01 2004-02-14 22:25:24 182 Threonine reduced to aminobutynate thr_amibut -17.002740 -17.0073 H(-1) O(-1) mdurham users 2004-02-12 20:43:37 2004-02-14 22:24:54 184 C13 label 13C9 9.030193 8.9339 C(-9) C13(9) hs users 2004-02-16 21:19:47 2004-11-06 16:46:26 185 C13 label (Phosphotyrosine) 13C9_Phospho_Tyr 88.996524 88.9138 H C(-9) C13(9) O(3) P hs users 2004-02-22 04:42:33 2004-02-28 21:03:12 188 C13 label 13C6 6.020129 5.9559 C(-6) C13(6) hs users 2004-02-28 19:54:10 2005-08-20 22:46:37 186 Hydroxyphenylglyoxal arginine Arg1HPG 132.021129 132.1162 H(4) C(8) O(2) gcarven users 2004-02-26 20:19:25 2004-02-28 15:02:17 187 2 Hydroxyphenylglyoxal arginine Arg2HPG 282.052823 282.2476 H(10) C(16) O(5) gcarven users 2004-02-26 22:05:25 2004-02-28 15:02:38 189 dimethyl labeling (D) Dimethyl-D 34.068961 34.0901 H(-2) H2(6) C(2) GapeHsu users 2004-03-09 03:59:08 2004-03-20 21:08:13 196 APTA d3 QATd3 174.168569 174.2784 H(16) H2(3) C(9) N(2) O s_julka users 2004-04-08 17:15:04 2005-02-14 11:56:01 193 O18 label at both C-terminal oxygens double_O18 4.008491 3.9995 O(-2) O18(2) Hensbergen users 2004-03-30 14:20:29 2004-04-05 14:11:33 194 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate AccQTag 170.048013 170.1674 H(6) C(10) N(2) O davidcreasy users 2004-04-08 12:01:19 2004-04-10 12:41:13 199 DiMethyl-CH2D CH2D 30.043854 30.0655 H(2) H2(2) C(2) PhilipHsu users 2004-04-20 07:07:29 2004-04-24 10:53:43 197 EAPTA d0 EQAT 184.157563 184.2786 H(20) C(10) N(2) O s_julka users 2004-04-08 17:21:08 2005-02-14 11:56:39 198 EAPTA d5 EQATd5 189.188947 189.3094 H(15) H2(5) C(10) N(2) O s_julka users 2004-04-08 17:25:24 2005-02-14 11:57:29 200 EDT EDT 75.980527 76.1838 H(4) C(2) O(-1) S(2) take users 2004-04-20 08:27:30 2004-04-24 10:54:54 202 APTA- d0 with no neutral loss QAT-NL 170.141913 170.2520 H(18) C(9) N(2) O s_julka users 2004-04-22 19:20:25 2004-04-24 10:51:25 203 APTA-d0 with quaternary amine loss QAT-QL 170.141913 170.2520 H(18) C(9) N(2) O s_julka users 2004-04-22 19:23:00 2005-02-14 11:57:54 212 N-ethyl iodoacetamide-d5 NEIAA-d5 90.084148 90.1353 H(2) H2(5) C(4) N O Botting users 2004-05-18 11:11:52 2004-08-27 16:55:49 205 Acrolein addition +94 Acrolein94 94.041865 94.1112 H(6) C(6) O fatcat users 2004-05-06 18:54:17 2004-11-06 16:58:07 206 Acrolein addition +56 Acrolein56 56.026215 56.0633 H(4) C(3) O fatcat users 2004-05-06 18:55:58 2004-11-06 16:57:26 207 Acrolein addition +38 Acrolein38 38.015650 38.0480 H(2) C(3) fatcat users 2004-05-06 18:57:08 2004-11-06 16:56:54 208 Acrolein addition +76 Acrolein76 76.031300 76.0960 H(4) C(6) fatcat users 2004-05-06 18:59:23 2004-11-06 16:57:44 209 Acrolein addition +112 Acrolein112 112.052430 112.1265 H(8) C(6) O(2) fatcat users 2004-05-06 19:00:25 2004-11-06 16:58:24 213 ADP Ribose addition to Arginine ADP-Ribose 541.061110 541.3005 H(21) C(15) N(5) O(13) P(2) ionjockey users 2004-05-27 20:53:37 2004-05-30 13:23:23 211 N-ethyl iodoacetamide-d0 NEIAA-d0 85.052764 85.1045 H(7) C(4) N O Botting users 2004-05-18 11:10:37 2004-08-27 16:55:26 214 Applied Biosystems iTRAQ(TM) multiplexed quantitation chemistry iTRAQ 144.102063 144.1544 H(12) C(4) C13(3) N N15 O Different channels have the same nominal mass but slightly different exact masses. The values used here are for iTRAQ116/7. iTRAQ114 is 0.0038 Da heavier, iTRAQ115 is 0.0025 Da lighter unimod admin 2004-06-08 14:04:07 2004-06-13 13:24:36 253 Crotonaldehyde Croton 70.041865 70.0898 H(6) C(4) O fatcat users 2004-08-12 19:56:03 2004-08-14 10:07:59 340 bromination of tryptophan bromo 77.910511 78.8961 H(-1) Br gerribe users 2005-09-06 08:31:51 2005-09-10 16:13:18 342 Tyrosine oxidation to 2-aminotyrosine aminotyrosine 15.010899 15.0146 H N unimod admin 2005-09-10 21:18:23 2005-09-10 23:02:52 343 oxidized Arginine biotinylated with biotin hydrazide Argbiotinhydrazide 199.066699 199.2700 H(13) C(9) N O(2) S unimod admin 2005-09-10 22:36:58 2005-09-10 23:03:17 257 Propionaldehyde +42 Propionald+42 42.046950 42.0797 H(6) C(3) fatcat users 2004-08-12 20:04:55 2004-11-06 17:00:49 258 O18 Labeling of Serine, Threonine or Tyrosine O18_STY 2.004246 1.9998 O(-1) O18 18O incorporation to serine, threonine, and tyrosine by using alkaline phosphatase to dephosphorylate chemgirl18 users 2004-08-27 21:57:17 2004-09-05 19:05:36 259 C13 and N15 label 13C6-15N2 8.014199 7.9427 C(-6) C13(6) N(-2) N15(2) hs01 users 2004-08-30 16:23:02 2004-11-06 16:45:52 260 Thiophosphorylation Thio-phospho 95.943487 96.0455 H O(2) P S mrbladergroen users 2004-09-01 13:20:58 2004-09-05 19:07:49 261 4-sulfophenyl isothiocyanate SPITC 214.971084 215.2495 H(5) C(7) N O(3) S(2) hatlevig users 2004-09-14 20:01:29 2004-09-17 19:00:29 243 label Cysteine with IGBP reagent Cysteine-Br 296.016039 297.1478 H(13) C(12) N(2) O(2) Br zqiao users 2004-07-26 23:02:04 2004-08-18 14:56:28 270 nucleophilic addtion to cytopiloyne cytopiloyne 362.136553 362.3738 H(22) C(19) O(7) Cytopiloyne is a polyacetylenic glucoside isolated form Bidens pilosa which can modulate T helper cell differentiation. Biotinylated cytopiloyne might be use to identify its receptor in T cell. ymchiang users 2004-11-10 03:13:23 2005-01-28 15:44:26 271 nucleophilic addition to cytopiloyne+H2O cytopiloyne+H2O 380.147118 380.3891 H(24) C(19) O(8) Cytopiloyne is a polyacetylenic glucoside isolated form Bidens pilosa which can modulated T helper cell differentiation. Biotinylated cytopiloyne might be use to identify its receptor in T cell. ymchiang users 2004-11-11 05:23:17 2005-01-28 15:45:48 267 C13 and N15 label 13C6-15N4 10.008269 9.9296 C(-6) C13(6) N(-4) N15(4) AFCS users 2004-10-20 17:49:33 2004-11-06 16:47:19 269 C13 and N15 label 13C9-15N1 10.027228 9.9273 C(-9) C13(9) N(-1) N15 Deepa users 2004-11-05 18:51:13 2004-11-06 16:48:06 262 Trideuteration D3_Label 3.018830 3.0185 H(-3) H2(3) mmolloy users 2004-09-29 08:52:01 2004-10-09 16:17:37 268 C13 and N15 label 13C5-15N1 6.013809 5.9567 C(-5) C13(5) N(-1) N15 Deepa users 2004-11-05 18:49:37 2004-11-06 16:52:39 264 phosphorylation to pyridyl thiol PET 121.035005 121.2028 H(7) C(7) N O(-1) S PET = 2-[4-pyridyl]ethanethiol vbad users 2004-10-07 08:55:33 2004-10-09 16:20:01 272 sulfonation of N-terminus CAF 135.983029 136.1265 H(4) C(3) O(4) S chens002 users 2004-11-16 14:25:33 2004-11-16 18:46:34 273 covalent modification of lysine by cross-linking reagent SSD_K 253.095023 253.2512 H(15) C(12) N O(5) tom users 2004-11-16 20:20:17 2004-11-21 13:00:14 274 hydroxy-methyl vinyl ketone HMVK 29.002740 29.0180 H C O nbarsh users 2004-12-04 20:22:03 2004-12-21 09:25:16 275 S-nitrosylation SNO 29.997989 30.0061 N O dengh users 2004-12-11 00:23:12 2004-12-11 11:02:05 276 Aminoethylbenzenesulfonylation AEBS 183.035399 183.2276 H(9) C(8) N O(2) S Potential protein modification when using AEBSF (Pefabloc) as a serine protease inhibitor. plattm users 2004-12-14 04:38:25 2004-12-18 16:59:31 277 Methyl methanethiosulfonate MMTS 45.987721 46.0916 H(2) C S unimod admin 2004-12-16 09:24:43 2005-01-15 18:34:47 278 Ethanolation of Cys EtOH 44.026215 44.0526 H(4) C(2) O A simplified procedure for the reduction and alkylation of cysteine residues in proteins prior to proteolytic digestion and mass spectral analysis. Hale JE, Butler JP, Gelfanova V, You JS, Knierman MD. Enabling Biology Division, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA. hale_john_e@lilly.com A procedure for reduction and alkylation of cysteine residues in proteins was developed using the volatile reagents triethylphosphine and iodoethanol. These reagents may be used to modify proteins in solution, as well as proteins in gel slices, prior to proteolytic digestion and mass spectral analysis. The procedure eliminates several steps with both types of samples. Samples in solution do not need to be desalted following reduction and alkylation, with excess reagent being removed under vacuum. For gel slices, the procedure combines washing, destaining, reduction and alkylation into a single step. The procedure was applied successfully to samples as complex as serum, and we demonstrated alkylation of cysteines to be quantitative in purified proteins. We also were able to reduce and alkylate proteins with these reagents during the gas phase. Elimination of the need for desalting of samples after reaction raised the possibility of automation of the procedure for liquid samples, which is difficult with conventional reduction and alkylation chemistries. knierman users 2005-01-11 04:26:47 2005-01-15 18:29:41 279 Cysteine sulfenic acid sulfenic_acid 15.994915 15.9994 O smdeva users 2005-01-13 22:21:04 2005-01-15 18:27:09 280 Ethylation Ethyl 28.031300 28.0532 H(4) C(2) Qishanl users 2005-01-27 23:18:07 2005-01-30 11:17:45 281 Cysteine modified Coenzyme A CysCoA 765.099560 765.5182 H(34) C(21) N(7) O(16) P(3) S tremis users 2005-02-01 17:10:00 2005-02-05 12:11:16 282 N-methylation N-Methyl 14.015650 14.0266 H(2) C Qishanl users 2005-02-08 17:51:05 2005-02-12 17:19:00 283 N-ethylation N-Ethyl 28.031300 28.0532 H(4) C(2) Qishanl users 2005-02-08 17:52:09 2005-02-12 17:19:53 284 Deuterium Methylation of Lysine DeMet 16.028204 16.0389 H2(2) C Glebivanov users 2005-02-13 01:26:38 2005-02-14 11:59:39 285 Light Sulfanilic Acid (SA) C12 SA_light 155.004099 155.1744 H(5) C(6) N O(2) S apanchaud users 2005-02-17 15:48:07 2005-07-11 14:50:37 286 Heavy Sulfanilic Acid (SA) C13 SA_heavy 161.024228 161.1303 H(5) C13(6) N O(2) S apanchaud users 2005-02-17 15:55:52 2005-07-11 14:49:22 289 Biotin polyethyleneoxide amine BiotinPEO-amine 356.188212 356.4835 H(28) C(16) N(4) O(3) S EDC crosslinker is used to couple biotin PEO-amine to carboxyl groups or 5' phosphate groups TBricker1 users 2005-03-02 16:49:17 2005-03-06 10:52:30 288 Tryptophan oxidation to oxolactone oxolactone 13.979265 13.9835 H(-2) O oxolactone users 2005-02-25 23:30:20 2005-02-26 16:31:02 290 Pierce EZ-Link Biotin-HPDP Biotin-HPDP 428.191582 428.6124 H(32) C(19) N(4) O(3) S(2) tgreco users 2005-03-03 22:56:52 2005-03-06 10:53:22 291 Mercury Mercaptan Hg 201.970617 200.5900 Hg tgreco users 2005-03-03 23:53:00 2005-03-06 10:54:52 292 Cross-link of (Iodo)-uracil MP with W,F,Y IodoU-AMP 322.020217 322.1654 H(11) C(9) N(2) O(9) P One note about this chemistry is that for W you have to take care of O2 big time (and extract the I-uracil monophosphate with organics to get rid of residual iodide). davidERICanderson users 2005-03-04 22:57:55 2005-03-06 10:56:25 293 3-(carbamidomethylthio)propanoyl CAMthiopropanoyl 145.019749 145.1796 H(7) C(5) N O(2) S vanschra users 2005-03-09 15:22:46 2005-03-13 21:01:46 294 biotinoyl-iodoacetyl-ethylenediamine ied-biotin 326.141261 326.4145 H(22) C(14) N(4) O(3) S alexey users 2005-03-10 16:28:09 2005-03-13 21:02:35 295 Fucose Fuc 146.057909 146.1412 H(10) C(6) O(4) rsack users 2005-03-22 16:57:00 2005-03-29 13:48:28 296 4-Sulfophenyl isocyante modification to N-term R TrypSPITCR 214.971084 215.2495 H(5) C(7) N O(3) S(2) nicolastaylor users 2005-03-23 15:33:41 2005-03-29 13:49:01 297 Guanidination of K followed by 4-Sulfophenyl isocyante modification to N-term K TrypGSPITCK 256.992883 257.2895 H(7) C(8) N(3) O(3) S(2) nicolastaylor users 2005-03-23 16:15:44 2005-03-29 13:49:34 298 deuterated methyl ester D3-Me-ester 17.034480 17.0451 H(-1) H2(3) C esterification of carboxylic acids using D3-Methanolic HCl ndacoyas users 2005-03-24 16:31:28 2005-06-15 16:26:14 299 Carboxylation carboxyl 43.989829 44.0095 C O(2) Carol users 2005-03-29 22:55:44 2005-04-02 18:18:10 300 Hydroxylethanone hydroxylethanone 58.005479 58.0361 H(2) C(2) O(2) Carol users 2005-03-29 23:01:21 2005-04-02 18:18:53 301 Monobromobimane derivative Bromobimane 190.074228 190.1986 H(10) C(10) N(2) O(2) catsriku users 2005-04-04 23:03:13 2005-04-09 11:58:17 302 Menadione derivative Menadione-Q 170.036779 170.1641 H(6) C(11) O(2) catsriku users 2005-04-04 23:21:29 2005-04-09 11:57:12 303 Cysteine mercaptoethanol DeStreak 75.998285 76.1176 H(4) C(2) O S harald users 2005-04-11 14:06:08 2005-04-16 18:19:59 305 Fucosylated biantennary (-2 galactose) G0 1444.533870 1445.3331 dHex Hex(3) HexNAc(4) whaskins users 2005-04-25 18:20:12 2005-04-30 11:29:25 306 Sulfitolysis Sulfitolysis 79.956815 80.0632 O(3) S whaskins users 2005-04-25 18:28:49 2005-04-30 11:20:43 307 Fucosylated biantennary (-1 galactose) G1 1606.586693 1607.4737 dHex Hex(4) HexNAc(4) whaskins users 2005-04-25 19:22:11 2005-04-30 11:33:35 308 Fucosylated biantennary G2 1768.639517 1769.6143 dHex Hex(5) HexNAc(4) whaskins users 2005-04-25 19:25:17 2005-04-30 11:35:20 309 Biantennary (-2 galactose) G0-Fuc 1298.475961 1299.1919 Hex(3) HexNAc(4) whaskins users 2005-04-25 19:27:51 2005-04-30 11:27:35 310 Biantennary (-1 galactose) G1-Fuc 1460.528784 1461.3325 Hex(4) HexNAc(4) whaskins users 2005-04-25 19:33:37 2005-04-30 11:32:22 311 Biantennary G2-Fuc 1622.581608 1623.4731 Hex(5) HexNAc(4) whaskins users 2005-04-25 19:35:54 2005-04-30 11:34:33 312 Cysteinylation Cys 120.011924 120.1502 H(6) C(3) N O(2) S whaskins users 2005-04-25 19:59:43 2005-04-30 11:21:35 313 C terminal -K from HC of MAb -K -128.094963 -128.1723 H(-12) C(-6) N(-2) O(-1) whaskins users 2005-04-25 20:05:38 2005-04-30 11:19:29 314 N-methylmaleimide NMM 111.032028 111.0987 H(5) C(5) N O(2) whaskins users 2005-04-25 20:12:24 2005-04-30 11:21:09 315 fluorescein-5-thiosemicarbazide FTC 421.073241 421.4259 H(15) C(21) N(3) O(5) S app95d users 2005-04-29 17:20:58 2005-04-30 11:37:33 316 2,5-dimethypyrrole pyrrole 78.046950 78.1118 H(6) C(6) Qishanl users 2005-05-07 03:11:23 2005-05-07 09:58:28 317 D-Succinimide D-Succ -18.010565 -18.0153 H(-2) O(-1) whaskins users 2005-05-09 21:09:20 2005-05-17 20:19:42 318 MDA adduct +62 MDA62 62.015650 62.0694 H(2) C(5) usually major adduct formed from malondialdehyde (MDA) with the amino group of lysine residues rkupfer users 2005-05-12 22:00:21 2005-05-21 10:32:02 319 MDA adduct +54 MDA54 54.010565 54.0474 H(2) C(3) O Malondialdehyde (MDA) adduct rkupfer users 2005-05-12 22:12:10 2005-05-21 10:31:41 320 N-ethylmaleimide hydrolysis NEM-hyd 143.058243 143.1406 H(9) C(6) N O(3) whaskins users 2005-05-16 22:18:42 2005-05-21 10:34:39 321 N-Succinimide N-Succ -17.002740 -17.0073 H(-1) O(-1) whaskins users 2005-05-17 20:18:38 2005-05-21 10:33:16 323 bis-N-I-sulfonerahodamine bNis 713.093079 713.5626 H(30) C(31) N(4) O(6) S I dengh users 2005-05-17 22:56:12 2005-05-21 10:43:02 324 DTBP DTBP 122.990948 123.6044 H(6) C(3) N S Cl takesin users 2005-05-18 08:43:26 2005-05-21 10:46:06 325 10-ethoxyphosphinyl-N-(biotinamidopentyl)decanamide FP-Biotin 573.323954 573.7485 H(50) C(27) N(4) O(5) P S FP-biotin was designed to label the active site serine of serine esterases/proteases. lmschopfer users 2005-05-19 22:01:37 2005-09-10 16:16:35 334 CAMthiopropanoyl of Lys DSP-iodoacetimide 146.027574 146.1875 H(8) C(5) N O(2) S job users 2005-08-09 21:36:32 2005-08-16 08:56:38 332 thiophosphate labeled with biotin-HPDP Thiophos-S-S-biotin 525.142894 525.6658 H(34) C(19) N(4) O(5) P S(3) lparker users 2005-07-21 16:37:10 2005-07-23 12:32:27 333 6-N-biotinylaminohexyl isopropyl phosphate Can-FP-biotin 448.203504 448.5371 H(35) C(19) N(3) O(5) P S Commercially available from Toronto Research Chemicals Inc, as of 2005. Designed to label the active site serine of serine esterases/proteases. lmschopfer users 2005-07-21 20:54:00 2005-09-10 16:15:48 341 threonine oxidation to 2-amino-3-oxo-butanoic acid 2-amino-3-oxo-butanoic_acid -2.015650 -2.0159 H(-2) unimod admin 2005-09-10 21:04:47 2005-09-10 23:02:31 335 reduced 4-Hydroxynonenal redHNE 158.130680 158.2380 H(18) C(9) O(2) Michael addition adduct of 4-hydroxynonenal with histidine, cystein and lysine residues stabilized by reduction with NaBH4 rkupfer users 2005-08-10 20:52:36 2005-08-16 08:58:03 361 oxidized Threonine biotinylated with biotin hydrazide Therbiotinhydrazide 240.104482 240.3252 H(16) C(10) N(4) O S unimod admin 2005-09-10 23:01:28 2005-09-10 23:12:52 337 Michael addition with methylamine MethylamineST 13.031634 13.0418 H(3) C N O(-1) zhulx users 2005-08-12 21:55:51 2005-08-16 08:55:00 18 308 P 1 17 308 O 4 16 308 H 3 15 307 P 1 14 307 O 4 13 307 H 3 33 404 N 1 32 404 C 3 31 404 H 9 23 402 H 6 24 402 H2 3 25 402 C 3 26 402 N 1 27 403 H 6 28 403 H2 3 29 403 C 3 30 403 N 1 34 405 H 9 35 405 C 3 36 405 N 1 37 406 H 3 38 406 H2 6 39 406 C 3 40 406 N 1 41 407 H 3 42 407 H2 6 43 407 C 3 44 407 N 1 45 408 H2 9 46 408 C 3 47 408 N 1 48 409 H2 9 49 409 C 3 50 409 N 1 414 1514 H 52 413 1512 S 1 412 1512 P 1 411 1512 O 6 410 1512 N 4 407 1511 S 1 406 1511 P 1 405 1511 O 6 404 1511 N 3 403 1511 C 19 401 1510 S 1 402 1511 H 37 400 1510 O 2 399 1510 N 3 398 1510 C 16 397 1510 H 28 396 1509 S 1 395 1509 P 1 394 1509 O 6 393 1509 N 3 392 1509 C 19 391 1509 H 37 315 1466 S 3 303 1464 S 3 314 1466 P 1 313 1466 O 5 312 1466 N 4 311 1466 C 19 310 1466 H 34 309 1465 S 3 308 1465 P 1 307 1465 O 5 306 1465 N 4 305 1465 C 19 304 1465 H 34 302 1464 P 1 301 1464 O 5 300 1464 N 4 299 1464 C 19 298 1464 H 34 408 1512 H 52 409 1512 C 27 418 1514 P 1 417 1514 O 6 416 1514 N 4 415 1514 C 27 419 1514 S 1 1 - 2 Anywhere 3 Any N-term 4 Any C-term 5 Protein N-term 6 Protein C-term 296 1 N-term 3 0 2 13 0.000000 0.0000 295 1 K 2 0 1 13 0.000000 0.0000 127 2 C-term 4 0 1 2 0.000000 0.0000 348 3 N-term 3 0 2 5 0.000000 0.0000 347 3 K 2 0 1 2 0.000000 0.0000 587 5 N-term 3 0 2 2 0.000000 0.0000 586 5 K 2 0 1 2 0.000000 0.0000 488 6 C 2 0 1 5 0.000000 0.0000 129 7 Q 2 0 1 2 0.000000 0.0000 128 7 N 2 0 1 2 0.000000 0.0000 548 8 C 2 1 1 11 0.000000 0.0000 549 9 C 2 1 1 11 0.000000 0.0000 114 10 M 4 0 1 5 0.000000 0.0000 266 11 M 4 0 1 5 0.000000 0.0000 1321 12 C 2 1 1 11 0.000000 0.0000 1320 13 C 2 1 1 11 0.000000 0.0000 274 14 S 2 1 3 2 0.000000 0.0000 273 14 E 2 0 2 2 0.000000 0.0000 163 15 N-term 5 0 1 2 0.000000 0.0000 153 16 N-term 5 0 1 2 0.000000 0.0000 712 17 C 2 0 1 5 0.000000 0.0000 145 18 C-term 4 0 1 11 0.000000 0.0000 289 19 W 2 0 2 6 0.000000 0.0000 288 19 M 2 0 1 6 0.000000 0.0000 287 19 H 2 0 2 6 0.000000 0.0000 264 20 C 2 0 1 5 0.000000 0.0000 314 22 T 2 1 5 2 0.000000 0.0000 313 22 S 2 1 4 2 0.000000 0.0000 312 22 H 2 1 3 2 0.000000 0.0000 311 22 D 2 1 2 2 0.000000 0.0000 310 22 C 2 1 1 2 0.000000 0.0000 269 23 Y 2 1 3 2 0.000000 0.0000 268 23 T 2 1 2 2 0.000000 0.0000 267 23 S 2 1 1 2 0.000000 0.0000 181 24 C 2 0 1 6 0.000000 0.0000 316 25 N-term 3 1 2 5 0.000000 0.0000 315 25 K 2 1 1 5 0.000000 0.0000 236 26 C 3 0 1 6 0.000000 0.0000 154 27 E 3 0 1 6 0.000000 0.0000 120 28 Q 3 0 1 6 0.000000 0.0000 318 29 N-term 3 0 2 5 0.000000 0.0000 317 29 K 2 0 1 5 0.000000 0.0000 291 30 D 2 0 2 6 0.000000 0.0000 192 31 C 2 0 1 5 0.000000 0.0000 160 32 M 2 0 1 2 0.000000 0.0000 270 33 R 2 1 1 2 0.000000 0.0000 281 34 Q 2 1 6 2 0.000000 0.0000 280 34 N-term 3 1 5 2 0.000000 0.0000 279 34 N 2 1 4 2 0.000000 0.0000 278 34 K 2 1 3 2 0.000000 0.0000 461 35 P 2 1 4 2 0.000000 0.0000 460 35 N 2 1 3 2 0.000000 0.0000 459 35 K 2 1 2 2 0.000000 0.0000 458 35 D 2 1 1 2 0.000000 0.0000 272 14 D 2 0 2 2 0.000000 0.0000 277 34 H 2 1 2 2 0.000000 0.0000 598 36 N-term 3 1 3 11 0.000000 0.0000 597 36 R 2 1 2 2 0.000000 0.0000 307 21 S 2 0 1 2 97.976896 97.9952 H(3) O(4) P 523 4 H 2 1 4 6 0.000000 0.0000 590 37 K 2 1 1 2 0.000000 0.0000 301 38 E 2 1 2 2 0.000000 0.0000 300 38 D 2 1 1 2 0.000000 0.0000 302 39 D 2 1 1 2 0.000000 0.0000 1227 40 Y 2 0 3 2 0.000000 0.0000 322 41 T 2 1 4 9 0.000000 0.0000 321 41 N-term 3 1 3 9 0.000000 0.0000 320 41 N 2 1 2 7 0.000000 0.0000 319 41 K 2 1 1 9 0.000000 0.0000 325 42 K 2 1 1 2 0.000000 0.0000 328 43 T 2 1 3 9 0.000000 0.0000 327 43 S 2 1 2 9 0.000000 0.0000 326 43 N 2 1 1 7 0.000000 0.0000 329 44 C 2 1 1 2 0.000000 0.0000 331 45 K 2 1 2 2 0.000000 0.0000 330 45 G 3 1 1 2 0.000000 0.0000 334 46 K 2 1 1 2 0.000000 0.0000 338 47 T 2 1 4 2 0.000000 0.0000 337 47 S 2 1 3 2 0.000000 0.0000 336 47 K 2 1 2 2 0.000000 0.0000 335 47 C 2 1 1 2 0.000000 0.0000 339 48 C 2 1 1 2 0.000000 0.0000 341 49 S 2 1 1 2 0.000000 0.0000 345 50 H 2 1 2 2 0.000000 0.0000 344 50 C 2 1 1 2 0.000000 0.0000 346 51 C 3 1 1 2 0.000000 0.0000 655 52 K 2 0 1 5 0.000000 0.0000 199 53 C 2 0 1 2 0.000000 0.0000 198 53 H 2 0 1 2 0.000000 0.0000 197 53 K 2 0 1 2 0.000000 0.0000 271 14 C-term 4 0 1 2 0.000000 0.0000 290 30 C-term 4 0 1 6 0.000000 0.0000 276 34 C 2 1 1 2 0.000000 0.0000 324 54 G 5 1 1 2 0.000000 0.0000 340 55 C 2 1 1 2 0.000000 0.0000 308 21 T 2 0 1 2 97.976896 97.9952 H(3) O(4) P 275 14 T 2 1 4 2 0.000000 0.0000 282 34 R 2 1 7 2 0.000000 0.0000 292 30 E 2 0 2 6 0.000000 0.0000 522 4 N-term 3 1 3 6 0.000000 0.0000 309 21 Y 2 0 2 2 0.000000 0.0000 323 41 W 2 1 5 9 0.000000 0.0000 379 56 N-term 3 1 2 11 0.000000 0.0000 378 56 K 2 1 1 11 0.000000 0.0000 381 57 N-term 3 1 2 11 0.000000 0.0000 380 57 K 2 1 1 11 0.000000 0.0000 383 58 N-term 3 1 2 11 0.000000 0.0000 382 58 K 2 1 1 11 0.000000 0.0000 385 59 N-term 3 1 2 11 0.000000 0.0000 384 59 K 2 1 1 11 0.000000 0.0000 402 61 K 2 1 1 11 62.092330 62.1287 H(6) H2(3) C(3) N 406 62 K 2 1 1 11 65.111160 65.1472 H(3) H2(6) C(3) N 408 63 K 2 1 1 11 68.129990 68.1657 H2(9) C(3) N 395 64 N-term 3 1 2 11 0.000000 0.0000 394 64 K 2 1 1 11 0.000000 0.0000 399 65 N-term 3 1 2 11 0.000000 0.0000 398 65 K 2 1 1 11 0.000000 0.0000 397 66 N-term 3 1 2 11 0.000000 0.0000 396 66 K 2 1 1 11 0.000000 0.0000 405 60 N-term 3 1 2 11 59.073499 59.1103 H(9) C(3) N 404 60 K 2 1 1 11 59.073499 59.1103 H(9) C(3) N 403 61 N-term 3 1 2 11 62.092330 62.1287 H(6) H2(3) C(3) N 407 62 N-term 3 1 2 11 65.111160 65.1472 H(3) H2(6) C(3) N 409 63 N-term 3 1 2 11 68.129990 68.1657 H2(9) C(3) N 1541 345 C 2 1 1 5 0.000000 0.0000 1511 333 S 2 1 1 5 466.214069 466.5523 H(37) C(19) N(3) O(6) P S 1513 325 Y 2 1 2 5 0.000000 0.0000 462 35 F 2 1 5 6 0.000000 0.0000 463 35 Y 2 1 6 6 0.000000 0.0000 1112 254 K 2 1 2 14 0.000000 0.0000 1028 253 K 2 1 3 14 0.000000 0.0000 1540 344 R 2 1 1 5 0.000000 0.0000 1538 342 Y 2 1 1 5 0.000000 0.0000 478 89 N-term 3 1 2 5 0.000000 0.0000 477 89 K 2 1 1 5 0.000000 0.0000 480 90 N-term 3 1 2 11 0.000000 0.0000 479 90 K 2 1 1 11 0.000000 0.0000 482 91 N-term 3 1 2 11 0.000000 0.0000 481 91 K 2 1 1 11 0.000000 0.0000 521 4 K 2 1 2 6 0.000000 0.0000 492 92 N-term 3 1 2 5 0.000000 0.0000 491 92 K 2 1 1 5 0.000000 0.0000 496 93 T 2 1 2 5 0.000000 0.0000 495 93 S 2 1 1 5 0.000000 0.0000 1085 94 K 2 1 1 11 0.000000 0.0000 1084 95 K 2 1 1 11 0.000000 0.0000 1537 341 T 2 1 1 5 0.000000 0.0000 503 97 C 2 1 1 11 0.000000 0.0000 1539 343 R 2 1 1 5 0.000000 0.0000 543 105 C 2 0 1 11 0.000000 0.0000 1027 253 H 2 1 2 14 0.000000 0.0000 520 4 C 2 0 1 5 0.000000 0.0000 519 4 E 2 1 6 6 0.000000 0.0000 518 4 D 2 1 5 6 0.000000 0.0000 547 106 C 2 0 1 11 0.000000 0.0000 554 107 N-term 5 1 1 4 only with Listeria monocytogenes (gram-positive bacteria) 0.000000 0.0000 546 108 C 2 1 1 5 0.000000 0.0000 575 112 K 2 1 1 5 0.000000 0.0000 907 215 N 2 1 1 7 0.000000 0.0000 581 119 C 2 1 1 5 0.000000 0.0000 574 113 K 2 1 1 5 0.000000 0.0000 577 114 P 2 1 1 5 0.000000 0.0000 576 115 P 2 1 1 5 0.000000 0.0000 572 116 R 2 1 1 5 0.000000 0.0000 573 117 R 2 1 1 5 0.000000 0.0000 585 118 T 2 1 2 5 0.000000 0.0000 584 118 S 2 1 1 5 0.000000 0.0000 588 5 R 2 1 3 6 0.000000 0.0000 589 5 C 2 1 4 6 0.000000 0.0000 591 37 R 2 1 2 5 0.000000 0.0000 613 125 N-term 3 1 2 2 0.000000 0.0000 596 36 K 2 1 1 2 0.000000 0.0000 608 123 C 2 1 1 11 0.000000 0.0000 609 124 C 2 1 1 11 0.000000 0.0000 703 122 N-term 3 1 2 5 0.000000 0.0000 704 122 K 2 1 1 5 May be seen when samples are incubated in strong formic acid (e.g. CNBr digest) 0.000000 0.0000 604 121 K 2 1 1 4 0.000000 0.0000 612 125 K 2 1 1 2 0.000000 0.0000 619 126 N-term 2 1 2 14 0.000000 0.0000 618 126 K 2 1 1 14 0.000000 0.0000 621 127 F 2 1 1 12 0.000000 0.0000 633 128 C 2 1 1 5 0.000000 0.0000 634 129 Y 2 1 1 5 0.000000 0.0000 636 130 Y 2 1 1 5 0.000000 0.0000 637 131 Y 2 1 1 5 0.000000 0.0000 1542 346 F 2 1 1 5 0.000000 0.0000 635 129 H 2 1 2 5 0.000000 0.0000 645 134 G 5 1 1 3 0.000000 0.0000 646 135 G 5 1 1 3 0.000000 0.0000 650 136 N-term 3 1 2 11 0.000000 0.0000 649 136 K 2 1 1 11 0.000000 0.0000 657 139 K 2 1 1 5 0.000000 0.0000 689 137 N 2 1 1 7 core complex 0.000000 0.0000 661 140 C-term 4 1 1 14 Virtual Modification for MS/MS of a-type ions 0.000000 0.0000 665 141 N-term 3 1 2 5 0.000000 0.0000 664 141 K 2 1 1 5 0.000000 0.0000 673 142 N 2 1 1 7 0.000000 0.0000 667 143 N 2 1 1 7 0.000000 0.0000 668 144 N 2 1 1 7 0.000000 0.0000 669 145 N 2 1 1 7 0.000000 0.0000 670 146 N 2 1 1 7 0.000000 0.0000 674 147 N 2 1 1 7 0.000000 0.0000 675 148 N 2 1 1 7 0.000000 0.0000 676 149 N 2 1 1 7 0.000000 0.0000 677 150 N 2 1 1 7 0.000000 0.0000 678 151 N 2 1 1 7 0.000000 0.0000 679 152 N 2 1 1 7 0.000000 0.0000 680 153 N 2 1 1 7 0.000000 0.0000 681 154 N 2 1 1 7 0.000000 0.0000 682 155 N 2 1 1 7 0.000000 0.0000 683 156 N 2 1 1 7 0.000000 0.0000 684 157 N 2 1 1 7 0.000000 0.0000 685 158 N 2 1 1 7 0.000000 0.0000 690 159 N 2 1 1 7 0.000000 0.0000 687 160 N 2 1 1 7 0.000000 0.0000 688 161 N 2 1 1 7 0.000000 0.0000 702 162 M 2 1 1 12 0.000000 0.0000 720 172 W 2 1 1 5 0.000000 0.0000 1425 327 S 2 1 1 5 0.000000 0.0000 717 170 N 2 1 1 7 0.000000 0.0000 721 171 W 2 1 1 5 0.000000 0.0000 811 193 C-term 4 1 1 11 0.000000 0.0000 757 176 H 2 1 2 2 Secondary adduct - much less common as C 0.000000 0.0000 756 176 K 2 1 1 2 Secondary adduct - much less common as C 0.000000 0.0000 755 176 C 2 1 3 2 Primary adduct formed 0.000000 0.0000 1099 184 Y 2 1 1 11 Used in SILAC experiment 0.000000 0.0000 765 178 T 2 1 2 5 0.000000 0.0000 764 178 S 2 1 1 5 0.000000 0.0000 761 179 S 2 1 1 8 0.000000 0.0000 760 180 T 2 1 1 8 0.000000 0.0000 763 181 S 2 1 1 8 0.000000 0.0000 762 182 T 2 1 1 8 0.000000 0.0000 800 185 Y 2 1 1 11 Used in SILAC experiment 0.000000 0.0000 778 186 R 2 1 1 5 0.000000 0.0000 779 187 R 2 1 1 5 0.000000 0.0000 1488 188 K 2 1 2 11 Used in SILAC experiment 0.000000 0.0000 806 189 N-term 3 1 2 5 0.000000 0.0000 805 189 K 2 1 1 5 0.000000 0.0000 821 194 N-term 3 1 2 5 0.000000 0.0000 820 194 K 2 1 1 5 0.000000 0.0000 1448 286 E 2 1 3 5 0.000000 0.0000 1447 286 D 2 1 2 5 0.000000 0.0000 1454 285 E 2 1 3 5 0.000000 0.0000 1237 198 C 2 1 1 11 0.000000 0.0000 1453 285 D 2 1 2 5 0.000000 0.0000 1236 197 C 2 1 1 5 0.000000 0.0000 850 199 N-term 3 1 2 5 0.000000 0.0000 849 199 K 2 1 1 5 0.000000 0.0000 852 200 T 2 1 2 5 0.000000 0.0000 851 200 S 2 1 1 5 0.000000 0.0000 1042 211 C 2 1 1 11 0.000000 0.0000 1234 195 C 2 1 1 5 0.000000 0.0000 1235 196 C 2 1 1 11 0.000000 0.0000 847 202 C 2 1 1 5 0.000000 0.0000 1452 285 C-term 4 1 1 5 0.000000 0.0000 1238 203 C 2 1 1 5 0.000000 0.0000 1109 205 K 2 1 1 14 0.000000 0.0000 1107 206 K 2 1 3 14 0.000000 0.0000 1104 207 K 2 1 1 14 0.000000 0.0000 1108 208 K 2 1 1 14 0.000000 0.0000 1110 209 K 2 1 1 14 0.000000 0.0000 1106 206 H 2 1 2 14 0.000000 0.0000 1105 206 C 2 1 1 14 0.000000 0.0000 1055 258 Y 2 1 3 11 0.000000 0.0000 876 213 R 2 1 1 9 0.000000 0.0000 1514 325 S 2 1 1 5 591.334518 591.7637 H(52) C(27) N(4) O(6) P S 1510 333 Y 2 1 2 5 326.190223 326.4774 H(28) C(16) N(3) O(2) S 1508 340 W 2 1 1 2 0.000000 0.0000 901 214 Y 2 0 3 11 Low abundance 0.000000 0.0000 902 214 N-term 3 0 2 11 0.000000 0.0000 900 214 K 2 0 1 11 0.000000 0.0000 1026 253 C 2 1 1 14 0.000000 0.0000 1114 255 K 2 1 2 14 0.000000 0.0000 1113 255 H 2 1 1 14 0.000000 0.0000 1116 256 K 2 1 2 14 0.000000 0.0000 1115 256 H 2 1 1 14 0.000000 0.0000 1118 257 K 2 1 2 14 0.000000 0.0000 1117 257 H 2 1 1 14 0.000000 0.0000 1111 254 H 2 1 1 14 0.000000 0.0000 1041 211 Y 2 1 2 11 0.000000 0.0000 1043 212 Y 2 1 2 11 0.000000 0.0000 1044 212 C 2 1 1 11 0.000000 0.0000 1036 243 C 2 1 1 5 0.000000 0.0000 1054 258 T 2 1 2 11 0.000000 0.0000 1053 258 S 2 1 1 11 0.000000 0.0000 1059 260 Y 2 1 3 14 0.000000 0.0000 1058 260 T 2 1 2 14 0.000000 0.0000 1057 260 S 2 1 1 14 0.000000 0.0000 1098 259 K 2 1 1 11 Used in SILAC experiment 0.000000 0.0000 1070 261 K 2 1 1 5 0.000000 0.0000 1071 261 N-term 3 1 2 5 0.000000 0.0000 1087 262 L 2 1 1 11 for SILAC expt 0.000000 0.0000 1103 268 V 2 1 1 11 Used in AQUA experiment 0.000000 0.0000 1089 264 T 2 1 2 5 0.000000 0.0000 1088 264 S 2 1 1 5 0.000000 0.0000 1174 273 K 2 1 1 5 0.000000 0.0000 1487 188 R 2 1 1 11 Used in SILAC experiment 0.000000 0.0000 1194 274 K 2 1 3 5 0.000000 0.0000 1100 267 R 2 1 1 11 Used in SILAC experiment 0.000000 0.0000 1101 269 F 2 1 1 11 Used in AQUA experiment 0.000000 0.0000 1211 270 Y 2 1 7 5 0.000000 0.0000 1210 270 S 2 1 6 5 0.000000 0.0000 1209 270 R 2 1 5 5 0.000000 0.0000 1208 270 P 2 1 4 5 0.000000 0.0000 1172 272 N-term 3 1 1 5 0.000000 0.0000 1218 271 Y 2 1 7 5 0.000000 0.0000 1217 271 T 2 1 6 5 0.000000 0.0000 1216 271 S 2 1 5 5 0.000000 0.0000 1215 271 R 2 1 4 5 0.000000 0.0000 1207 270 N-term 3 1 3 5 0.000000 0.0000 1206 270 K 2 1 2 5 0.000000 0.0000 1205 270 C 2 1 1 5 0.000000 0.0000 1214 271 N-term 3 1 3 5 0.000000 0.0000 1213 271 K 2 1 2 5 0.000000 0.0000 1212 271 C 2 1 1 5 0.000000 0.0000 1193 274 H 2 1 2 5 0.000000 0.0000 1192 274 C 2 1 1 5 0.000000 0.0000 1179 275 C 2 1 1 2 0.000000 0.0000 1189 276 S 2 1 4 6 Primary site of modification 0.000000 0.0000 1188 276 N-term 5 1 3 6 0.000000 0.0000 1187 276 K 2 1 2 6 0.000000 0.0000 1186 276 H 2 1 1 6 0.000000 0.0000 1200 277 C 2 0 1 5 Used with Applied Biosystems iTRAQ(TM) tags 0.000000 0.0000 1190 276 Y 2 1 5 6 0.000000 0.0000 1199 278 C 2 0 1 5 from reaction of SH group with iodoethanol 0.000000 0.0000 1198 279 C 2 1 1 5 0.000000 0.0000 1220 280 N-term 3 1 2 13 0.000000 0.0000 1219 280 K 2 1 1 13 0.000000 0.0000 1228 281 C 2 1 1 2 0.000000 0.0000 1226 40 T 2 0 2 2 0.000000 0.0000 1225 40 S 2 0 1 2 0.000000 0.0000 1231 282 N-term 5 1 1 5 0.000000 0.0000 1232 283 N-term 5 1 1 5 0.000000 0.0000 1239 284 K 2 1 1 11 0.000000 0.0000 1275 289 E 2 1 3 5 EDC-coupled modification of D, E and C-terminus 0.000000 0.0000 1263 288 W 2 1 1 5 0.000000 0.0000 1446 286 C-term 4 1 1 5 0.000000 0.0000 1274 289 D 2 1 2 5 EDC-coupled modification of D, E and C-terminus 0.000000 0.0000 1273 289 C-term 6 1 1 5 EDC-coupled modification of D, E and C-terminus 0.000000 0.0000 1276 290 C 2 1 1 5 0.000000 0.0000 1277 291 C 2 1 1 5 0.000000 0.0000 1279 292 W 2 1 2 5 Used in base/residue interactions (observed) 0.000000 0.0000 1278 292 F 2 1 1 5 Used in base/residue interactions (in principle?) 0.000000 0.0000 1280 292 Y 2 1 3 5 Used in base/residue interactions (in principle?) 0.000000 0.0000 1285 293 N-term 5 1 2 5 0.000000 0.0000 1284 293 K 2 1 1 5 0.000000 0.0000 1286 294 C 2 1 1 5 0.000000 0.0000 1309 295 T 2 1 2 8 0.000000 0.0000 1308 295 S 2 1 1 8 0.000000 0.0000 1310 296 R 3 1 1 5 Modification of tryptic peptides 0.000000 0.0000 1311 297 K 3 1 1 5 Modification of tryptic peptides 0.000000 0.0000 1422 298 E 2 1 3 11 0.000000 0.0000 1421 298 D 2 1 2 11 0.000000 0.0000 1420 298 C-term 2 1 1 11 0.000000 0.0000 1318 299 W 2 1 1 5 0.000000 0.0000 1319 300 W 2 1 1 5 0.000000 0.0000 1342 301 C 2 1 1 5 0.000000 0.0000 1341 302 K 2 1 2 5 0.000000 0.0000 1340 302 C 2 1 1 5 0.000000 0.0000 1347 303 C 2 1 1 5 0.000000 0.0000 1373 306 C 2 1 1 5 0.000000 0.0000 1377 305 N 2 1 1 7 0.000000 0.0000 1380 307 N 2 1 1 7 0.000000 0.0000 1382 308 N 2 1 1 7 0.000000 0.0000 1376 309 N 2 1 1 7 0.000000 0.0000 1379 310 N 2 1 1 7 0.000000 0.0000 1381 311 N 2 1 1 7 0.000000 0.0000 1375 312 C 2 1 1 13 0.000000 0.0000 1372 313 K 6 1 1 2 0.000000 0.0000 1374 314 C 2 1 1 5 0.000000 0.0000 1387 315 S 2 1 5 5 0.000000 0.0000 1386 315 R 2 1 4 5 0.000000 0.0000 1385 315 P 2 1 3 5 0.000000 0.0000 1384 315 K 2 1 2 5 0.000000 0.0000 1383 315 C 2 1 1 5 0.000000 0.0000 1389 316 K 2 1 1 5 0.000000 0.0000 1400 317 D 2 1 1 5 0.000000 0.0000 1409 318 K 2 1 1 5 0.000000 0.0000 1408 319 K 2 1 1 5 0.000000 0.0000 1411 320 C 2 1 1 5 0.000000 0.0000 1410 321 N 2 1 1 5 0.000000 0.0000 1435 329 R 2 1 1 11 0.000000 0.0000 1412 323 C 2 1 1 5 0.000000 0.0000 1416 324 R 2 1 3 5 0.000000 0.0000 1415 324 Q 2 1 2 5 0.000000 0.0000 1414 324 N 2 1 1 5 0.000000 0.0000 1512 325 T 2 1 3 5 591.334518 591.7637 H(52) C(27) N(4) O(6) P S 1436 330 R 2 1 1 11 0.000000 0.0000 1466 332 Y 2 1 3 5 525.142894 525.6658 H(34) C(19) N(4) O(5) P S(3) 1465 332 T 2 1 2 5 525.142894 525.6658 H(34) C(19) N(4) O(5) P S(3) 1464 332 S 2 1 1 5 525.142894 525.6658 H(34) C(19) N(4) O(5) P S(3) 1509 333 T 2 1 3 5 466.214069 466.5523 H(37) C(19) N(3) O(6) P S 1483 334 K 2 1 1 5 proteins are treated with DSP followed by DTT and iodoacetimide 0.000000 0.0000 1486 335 K 2 1 3 5 0.000000 0.0000 1485 335 H 2 1 2 5 0.000000 0.0000 1484 335 C 2 1 1 5 0.000000 0.0000 1489 188 L 2 1 3 11 Used in SILAC experiment 0.000000 0.0000 1481 337 S 2 1 1 6 0.000000 0.0000 1482 337 T 2 1 2 6 0.000000 0.0000 1490 188 F 2 1 4 11 Used in SILAC experiment 0.000000 0.0000 1543 347 W 2 1 1 5 0.000000 0.0000 1544 348 H 2 1 1 5 0.000000 0.0000 1545 349 H 2 1 1 5 0.000000 0.0000 1546 350 W 2 1 1 5 0.000000 0.0000 1547 351 W 2 1 1 5 0.000000 0.0000 1548 352 K 2 1 1 5 0.000000 0.0000 1549 353 K 2 1 1 5 0.000000 0.0000 1551 354 Y 2 1 2 5 0.000000 0.0000 1550 354 W 2 1 1 5 0.000000 0.0000 1552 355 Q 2 1 1 5 0.000000 0.0000 1553 356 Q 2 1 1 5 0.000000 0.0000 1554 357 P 2 1 1 5 0.000000 0.0000 1555 358 P 2 1 1 5 0.000000 0.0000 1556 359 P 2 1 1 5 0.000000 0.0000 1557 360 P 2 1 1 5 0.000000 0.0000 1558 361 T 2 1 1 5 0.000000 0.0000 1 - 2 PubMed PMID 3 Medline MUID 4 CAS Registry 5 GlycoSuiteDB 6 CarbBank 7 RESID 8 Swiss-Prot 9 Prosite 10 PIR 11 Entrez 12 Book 13 Journal 14 Misc. URL 15 Other 1 27 13 Miller et.al. Arch. Biochem. Biophy. (1993) 301, 41-52. 7 26 13 J. Proteome Res. 1, 181-187 (2002) 16 39 13 J. A. Kowalak and K. A. Walsh, Protein Science (1996) 5: 1625-1632 4 53 2 11327326 5 53 13 Poli G, Schaur RJ., IUBMB Life 2000 Oct-Nov;50(4-5):315-21 19 54 7 AA0058 18 54 2 7398618 17 54 13 Lin, T.S. Kolattukudy, P.E., Eur. J. Biochem. 106, 341-351, 1980 146 17 13 Galvani et.al. (2000) Electrophoresis vol 22 p2046-p2074 20 55 7 AA0229 49 56 13 Comparative proteomics based on stable isotope labeling and affinity selection. Regnier, Fred E.; Riggs, Larry; Zhang, Roujian; Xiong, Li; Liu, Peiran; Chakraborty, Asish; Seeley, Erin; Sioma, Cathy; Thompson, Robert A. Department of Chemistry, Pu 48 56 13 Global internal standard technology for comparative proteomics. Chakraborty, Asish; Regnier, Fred E.. Department of Chemistry, Purdue University, West Lafayette, IN, USA. Journal of Chromatography, A (2002), 949(1-2), 173-184. 47 56 13 Controlling Deuterium isotope effects in comparative proteomics. Zhang, Roujian; Sioma, Cathy S.; Thompson, Robert A.; Xiong, Li; Regnier, Fred E.. Department of Chemistry, Purdue University, West Lafayette, IN, USA. Analytical Chemistry (2 52 57 13 Controlling Deuterium isotope effects in comparative proteomics. Zhang, Roujian; Sioma, Cathy S.; Thompson, Robert A.; Xiong, Li; Regnier, Fred E.. Department of Chemistry, Purdue University, West Lafayette, IN, USA. Analytical Chemistry (2 51 57 13 Global internal standard technology for comparative proteomics. Chakraborty, Asish; Regnier, Fred E.. Department of Chemistry, Purdue University, West Lafayette, IN, USA. Journal of Chromatography, A (2002), 949(1-2), 173-184. 50 57 13 Comparative proteomics based on stable isotope labeling and affinity selection. Regnier, Fred E.; Riggs, Larry; Zhang, Roujian; Xiong, Li; Liu, Peiran; Chakraborty, Asish; Seeley, Erin; Sioma, Cathy; Thompson, Robert A. Department of Chemistry, Pu 55 58 13 Controlling Deuterium isotope effects in comparative proteomics. Zhang, Roujian; Sioma, Cathy S.; Thompson, Robert A.; Xiong, Li; Regnier, Fred E.. Department of Chemistry, Purdue University, West Lafayette, IN, USA. Analytical Chemistry (2 54 58 13 Global internal standard technology for comparative proteomics. Chakraborty, Asish; Regnier, Fred E.. Department of Chemistry, Purdue University, West Lafayette, IN, USA. Journal of Chromatography, A (2002), 949(1-2), 173-184. 53 58 13 Comparative proteomics based on stable isotope labeling and affinity selection. Regnier, Fred E.; Riggs, Larry; Zhang, Roujian; Xiong, Li; Liu, Peiran; Chakraborty, Asish; Seeley, Erin; Sioma, Cathy; Thompson, Robert A. Department of Chemistry, Pu 58 59 13 Controlling Deuterium isotope effects in comparative proteomics. Zhang, Roujian; Sioma, Cathy S.; Thompson, Robert A.; Xiong, Li; Regnier, Fred E.. Department of Chemistry, Purdue University, West Lafayette, IN, USA. Analytical Chemistry (2 57 59 13 Global internal standard technology for comparative proteomics. Chakraborty, Asish; Regnier, Fred E.. Department of Chemistry, Purdue University, West Lafayette, IN, USA. Journal of Chromatography, A (2002), 949(1-2), 173-184. 56 59 13 Comparative proteomics based on stable isotope labeling and affinity selection. Regnier, Fred E.; Riggs, Larry; Zhang, Roujian; Xiong, Li; Liu, Peiran; Chakraborty, Asish; Seeley, Erin; Sioma, Cathy; Thompson, Robert A. Department of Chemistry, Pu 71 60 13 Comparative proteomics based on stable isotope labeling and affinity selection. Regnier, Fred E.; Riggs, Larry; Zhang, Roujian; Xiong, Li; Liu, Peiran; Chakraborty, Asish; Seeley, Erin; Sioma, Cathy; Thompson, Robert A. Department of Chemistry, Pu 70 61 13 Comparative proteomics based on stable isotope labeling and affinity selection. Regnier, Fred E.; Riggs, Larry; Zhang, Roujian; Xiong, Li; Liu, Peiran; Chakraborty, Asish; Seeley, Erin; Sioma, Cathy; Thompson, Robert A. Department of Chemistry, Pu 72 62 13 Comparative proteomics based on stable isotope labeling and affinity selection. Regnier, Fred E.; Riggs, Larry; Zhang, Roujian; Xiong, Li; Liu, Peiran; Chakraborty, Asish; Seeley, Erin; Sioma, Cathy; Thompson, Robert A. Department of Chemistry, Pu 73 63 13 Comparative proteomics based on stable isotope labeling and affinity selection. Regnier, Fred E.; Riggs, Larry; Zhang, Roujian; Xiong, Li; Liu, Peiran; Chakraborty, Asish; Seeley, Erin; Sioma, Cathy; Thompson, Robert A. Department of Chemistry, Pu 64 64 13 Controlling Deuterium isotope effects in comparative proteomics. Zhang, Roujian; Sioma, Cathy S.; Thompson, Robert A.; Xiong, Li; Regnier, Fred E.. Department of Chemistry, Purdue University, West Lafayette, IN, USA. Analytical Chemistry (2 63 64 13 Comparative proteomics based on stable isotope labeling and affinity selection. Regnier, Fred E.; Riggs, Larry; Zhang, Roujian; Xiong, Li; Liu, Peiran; Chakraborty, Asish; Seeley, Erin; Sioma, Cathy; Thompson, Robert A. Department of Chemistry, Pu 68 65 13 Controlling Deuterium isotope effects in comparative proteomics. Zhang, Roujian; Sioma, Cathy S.; Thompson, Robert A.; Xiong, Li; Regnier, Fred E.. Department of Chemistry, Purdue University, West Lafayette, IN, USA. Analytical Chemistry (2 67 65 13 Comparative proteomics based on stable isotope labeling and affinity selection. Regnier, Fred E.; Riggs, Larry; Zhang, Roujian; Xiong, Li; Liu, Peiran; Chakraborty, Asish; Seeley, Erin; Sioma, Cathy; Thompson, Robert A. Department of Chemistry, Pu 66 66 13 Controlling Deuterium isotope effects in comparative proteomics. Zhang, Roujian; Sioma, Cathy S.; Thompson, Robert A.; Xiong, Li; Regnier, Fred E.. Department of Chemistry, Purdue University, West Lafayette, IN, USA. Analytical Chemistry (2 65 66 13 Comparative proteomics based on stable isotope labeling and affinity selection. Regnier, Fred E.; Riggs, Larry; Zhang, Roujian; Xiong, Li; Liu, Peiran; Chakraborty, Asish; Seeley, Erin; Sioma, Cathy; Thompson, Robert A. Department of Chemistry, Pu 77 4 13 Creasy, D. M., Cottrell, J. S., Proteomics 2 1426-34 (2002) 76 4 13 Boja, E. S., Fales, H. M., Anal. Chem. 73 3576-82 (2001) 89 105 14 http://www.appliedbiosystems.com/products/productdetail.cfm?prod_id=153 93 106 14 http://www.appliedbiosystems.com/products/productdetail.cfm?prod_id=153 96 107 2 10825024 97 107 2 8758896 102 37 13 J. Hirota et. al., Rapid Commun. Mass Spectrom., 17 371-6 (2003) 101 119 13 JBiolChem 2002 vol277 pp17048-17056 106 123 13 Silverman, Harbury, J. Biol. Chem. 2002, 277, 34, pp. 30968-30975 107 124 13 Silverman, Harbury, J. Biol. Chem. 2002, 277, 34, pp. 30968-30975 112 126 13 Lee,W.T. and Conrad, D.H. 1985, J. Immunol. 134:518 113 126 13 Lomant, A.J. and Fairbanks, G., J.Mol. Biol. 104, 243-261 (1976) 114 128 13 Gorman, J. J., Anal Biochem 1984, 160, 376 115 128 13 Gorman, J. J., Eur. J. Biochem 1987, 168-9 122 134 2 Neubert TA, Johnson RS, Hurley JB, Walsh KA (1992). The rod transducin alpha subunit amino terminus is heterogeneously fatty acylated. J Biol Chem. 267(26):18274-7. 123 135 2 Neubert TA, Johnson RS, Hurley JB, Walsh KA (1992). The rod transducin alpha subunit amino terminus is heterogeneously fatty acylated. J Biol Chem. 267(26):18274-7. 125 136 13 Julka,S.Regnier,F.E J.Chromatography Submitted 137 137 15 Oxford Glycosystems Tools for Glycobiology 132 139 4 dansyl chloride [605-65-2] 130 52 13 Anal. Biochem. 287, p110-117, 2000 136 140 13 D. Suckau, A. Resemann 2003 Anal. Chem., in press 291 327 15 2005 ASMS poster Presentation TP 527 151 170 2 Anal. Biochem. 1992, 205:151-158 145 17 13 Krutzsch and Inman, Analytical Biochemistry, 209, 109-116, (1993) 155 171 13 Rapid Communications in Mass Spectrometry, 17, 1642-1650 (2003) 154 172 13 Rapid Communications in Mass Spectrometry, 17, 1642-1650 (2003) 177 194 13 Hochleitner, E. O. et al, Proteomics 2004, 4, 669-676, 165 185 13 Ong, S-E, I. Kratchmarova, and M. Mann (2003). J Proteome Research 2: 173-181 321 188 13 Ong, S-E, I. Kratchmarova, and M. Mann (2003). J Proteome Research 2: 173-181 252 272 13 Anal.Chem 75(7):156A-165A 250 184 13 Ong, S-E, I. Kratchmarova, and M. Mann (2003). J Proteome Research 2: 173-181 167 189 2 Anal Chem 2003. 75, 6843 186 214 14 http://docs.appliedbiosystems.com/pebiodocs/04351918.pdf 176 194 15 Waters Application Note 930494 368 356 13 Berlett, Barbara S.; Stadtman, Earl R. Journal of Biological Chemistry (1997), 272(33), 20313-20316. 365 353 13 Yoo, Byoung-Sam; Regnier, Fred E. Electrophoresis (2004), 25(9), 1334-1341 366 354 13 Berlett, Barbara S.; Stadtman, Earl R. Journal of Biological Chemistry (1997), 272(33), 20313-20316. 367 355 13 Berlett, Barbara S.; Stadtman, Earl R. Journal of Biological Chemistry (1997), 272(33), 20313-20316. 364 352 13 Berlett, Barbara S.; Stadtman, Earl R. Journal of Biological Chemistry (1997), 272(33), 20313-20316. 363 351 13 Berlett, Barbara S.; Stadtman, Earl R. Journal of Biological Chemistry (1997), 272(33), 20313-20316. 329 340 2 Jimenez, E. C., Craig, A. G., Watkins, M., Hillyard, D. R., Gray, W. R., Gulyas, J., Rivier, J. E., Cruz, L. J. and Olivera, B. M. (1997). Bromocontryphan: post-translational bromination of tryptophan. Biochemistry 36, 989-994. 353 341 13 Berlett, Barbara S.; Stadtman, Earl R. Journal of Biological Chemistry (1997), 272(33), 20313-20316. 354 342 13 Berlett, Barbara S.; Stadtman, Earl R. Journal of Biological Chemistry (1997), 272(33), 20313-20316. 355 343 13 Yoo, Byoung-Sam; Regnier, Fred E. Electrophoresis (2004), 25(9), 1334-1341. 356 344 13 Berlett, Barbara S.; Stadtman, Earl R. Journal of Biological Chemistry (1997), 272(33), 20313-20316. 357 345 13 Berlett, Barbara S.; Stadtman, Earl R. Journal of Biological Chemistry (1997), 272(33), 20313-20316. 358 346 13 Berlett, Barbara S.; Stadtman, Earl R. Journal of Biological Chemistry (1997), 272(33), 20313-20316. 359 347 13 Berlett, Barbara S.; Stadtman, Earl R. Journal of Biological Chemistry (1997), 272(33), 20313-20316. 360 348 13 Berlett, Barbara S.; Stadtman, Earl R. Journal of Biological Chemistry (1997), 272(33), 20313-20316. 361 349 13 Berlett, Barbara S.; Stadtman, Earl R. Journal of Biological Chemistry (1997), 272(33), 20313-20316. 362 350 13 Berlett, Barbara S.; Stadtman, Earl R. Journal of Biological Chemistry (1997), 272(33), 20313-20316. 231 243 15 Target discovery , Inc. IDBEST IGBP user manual 242 261 13 Wang et al. 2004. Rapid Commun Mass Spectrom. 18(1):96-102 247 94 14 http://www.chem.agilent.com/cag/lystag.asp 245 95 14 http://www.chem.agilent.com/cag/lystag.asp 246 95 13 http://dx.doi.org/10.1002/rcm.517 248 94 13 http://dx.doi.org/10.1002/rcm.517 254 273 13 Partis MD et al. 1983 J Prot Chem 2 263-277 262 278 2 Anal Biochem. 2004 Oct 1;333(1):174-81 258 276 13 Biochim Biophys Acta. 1996 Feb 16; 1299(3):353-7 263 277 15 Applied Biosystems iTRAQ(TM) Reagents Chemistry Reference Guide Part Number 4351918 Rev. A 267 40 13 Medzihradszky et al., Mol. Cell. Proteomics 2004, 3, 429-440 269 284 13 Nature. 2004 Nov 18;432(7015):353-60. Epub 2004 Nov 03 271 289 15 Pierce Chemical Co. Handbook and Catalog 275 293 13 Peirce MJ, Wait R, Begum S, Saklatvala J, Cope AP (2004) Mol Cell Proteomics 3: 56-65 274 293 13 Swaim CL, Smith JB, Smith DL (2004) J Am Soc Mass Spectrom 15:736-749 281 297 13 Chen et al. (2004) Rapid Communications in Mass Spectrometry 18 96-102 280 296 13 Chen et al. (2004) Rapid Communications in Mass Spectrometry 18 96-102 283 315 2 Ahn, B., Rhee, S.G., and Stadtmann, E. R. (1987) Anal. Biochem. 286 319 13 Uchida K, Sakai K, Itakura K, Osawa T, Toyokuni S. 1977. Protein modification by lipid peroxidation products: formation of malondialdehyde-derived N(epsilon)-(2-propenol)lysine in proteins. Arch Biochem Biophys. 346(1):45-52 331 325 13 Liu, Y., Patricelli, M.P. & Cravatt, B.F. Activity-based protein profiling: The serine hydrolases. Proc Natl Acad Sci USA 96, 14694 (1999) 301 329 2 Identifying and quantifying in vivo methylation sites by heavy methyl SILAC, Shao-En Ong et al., Nature methods 1(2) 2004, 1-8 302 330 2 Identifying and quantifying in vivo methylation sites by heavy methyl SILAC, Shao-En Ong et al., Nature Methods 1(2) 1-8 307 285 15 ASMS 2005: Panchaud et al. (Poster ThP 509) ''Combining protein identification and quantitation: C-terminal isotope-coded tagging'' 305 286 15 ASMS 2005: Panchaud et al. (Poster ThP 509) ''Combining protein identification and quantitation: C-terminal isotope-coded tagging'' 330 333 13 Higson etal Synthesis 3, 407 (1999) 320 335 2 Crabb JW et al, Protein Sci. 2002;11: 831 319 337 13 Rapid Commun. Mass Spectrom. 2002; 16: 999-1001 369 357 13 Yoo, Byoung-Sam; Regnier, Fred E. Electrophoresis (2004), 25(9), 1334-1341 370 358 13 Berlett, Barbara S.; Stadtman, Earl R. Journal of Biological Chemistry (1997), 272(33), 20313-20316. 371 359 13 Berlett, Barbara S.; Stadtman, Earl R. Journal of Biological Chemistry (1997), 272(33), 20313-20316. 372 360 13 Berlett, Barbara S.; Stadtman, Earl R. Journal of Biological Chemistry (1997), 272(33), 20313-20316. 373 361 13 Yoo, Byoung-Sam; Regnier, Fred E. Electrophoresis (2004), 25(9), 1334-1341